NDLI: Determination of glucose in human serum based on an onion primary cuticula biosensor immobilized glucose oxidase

Content Provider	Royal Society of Chemistry (RSC)
Author	Shuang, Shaomin Dong, Chuan Choi, Martin M. F. Liu, Wenjuan Cui, Miao Jia, Wenjuan Zhang, Yan
Copyright Year	2012
Abstract	A glucose biosensor based on onion primary cuticula immobilized glucose oxidase has been developed. The glucose concentration was quantified by the decrease of dissolved oxygen. The effects of enzyme loading, pH, temperature, buffer concentration and interference have been studied in detail. The biosensor showed a wide linear relationship with a concentration range of 20 μM–1.1 mM, a fast response time (70 s), good repeatability (2.8%, n = 10) and satisfactory reproducibility (0.91%, n = 3). The biosensor retained 90% of its initial response after 3 months at 25 °C. The apparent Michaelis-Menten constant Km was estimated to be 0.042 mM, which revealed higher biological affinity to glucose. The proposed biosensor method has been successfully applied to the determination of glucose concentration in human blood serum and obtained results were agreement with the colorimetric assay kit. The recoveries of proposed method are 95.2–106.3%.
Starting Page	1432
Ending Page	1437
Page Count	6
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	4
Issue Number	5
Journal	Analytical Methods
DOI	10.1039/c2ay05945b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Glucose oxidase Oxygen Onion Enzyme Biosensor Michaelis\u2013Menten kinetics PH Cuticle Glucose Blood plasma
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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