NDLI: Screening target components from <i>Radix salviae miltiorrhiae</i> using an EGFR/CMC-online-HPLC/MS method

Content Provider	Royal Society of Chemistry (RSC)
Author	Hu, Zhi-Gang Wang, Si-Cen Han, Sheng-Li Huang, Jing Zhang, Tao
Copyright Year	2012
Abstract	Radix salviae miltiorrhizae is a widely used traditional Chinese medicine, it is the root and rhizome of Salvia miltiorrhiza Bge and belongs to the family Labiatae. Many of its pharmacological effects have been reported in a previous study. In this study, a two-dimension online analytical method of epidermal growth factor receptor cell membrane chromatography combined with liquid chromatography/mass spectrometry was built for recognition, separation and identification of active components from traditional Chinese herb Radix salviae miltiorrhizae. Gefitinib was used as positive control, while atenolol, nicotine, and nimodipine were used as negative controls to investigate the selectivity of the EGFR/CMC column and system suitability of this online analytical method. Cryptotanshinone was screened from Radix salviae miltiorrhizae by this online analytical method. Through in vitro pharmacological trials, cryptotanshinone showed obvious and dose-dependent inhibition activity in the range 0.40 to 50.0 μM. Molecular docking assay showed that the cryptotanshinone binding region was in very good agreement with gefitinib. The EGFR/CMC-online-LC/MS two-dimension analytical method was demonstrated as an effective screening method that rapidly recognizes and enriches active components acting on the epidermal growth factor receptor from Radix salviae miltiorrhizae and separates and identifies them.
Starting Page	1078
Ending Page	1083
Page Count	6
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	4
Issue Number	4
Journal	Analytical Methods
DOI	10.1039/c2ay05857j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Bge Estrogen receptor Cryptotanshinone Cell membrane Traditional Chinese medicine Lamiaceae Atenolol Chromatography Epidermal growth factor receptor Salvia miltiorrhiza Nimodipine Rhizome Salvia Gefitinib Mass spectrometry Growth factor Nicotine
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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