NDLI: Quantitative monitoring of the progress of organic reactions using multivariate image analysis-thin layer chromatography (MIA-TLC) method

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Analytical Methods Vol - 4 Issue - 3

Analytical Methods Vol - 4 Issue - 2

Analytical Methods Vol -3

Analytical Methods Vol -2

Analytical Methods Vol -1

Quantitative monitoring of the progress of organic reactions using multivariate image analysis-thin layer chromatography (MIA-TLC) method

Content Provider	Royal Society of Chemistry (RSC)
Author	Akhond, Morteza Hemmateenejad, Bahram Mobaraki, N. Mohammadpour, Zahra
Copyright Year	2012
Abstract	A thin layer chromatographic method combined with an image processing system (MIA-TLC) was developed for quantitative monitoring of the progress of organic reactions. Two simple reactions including alkaline hydrolysis of phenyl benzoate and reduction of benzaldehyde derivatives were chosen as model reactions. Small aliquots of starting material as well as successive aliquots of the reaction mixture in the course of the reaction were spotted on the TLC plate by micropipette. Hexane–ethyl acetate (with volume ratios of 15 : 1 and 3 : 1 for alkaline hydrolysis and reduction reactions, respectively) was chosen as the mobile phase. After TLC development, images of the plates were recorded using a home-made imaging cabinet. A home-made program was able to covert image data into three and two dimensional chromatograms, from which changes in the amount of reactants as a function of reaction time was easily monitored. By analysis of obtained chromatograms as a function of reaction time we calculated the conversion percentage of the reactions. The conversion percentage for both reactions was almost 99.9%. Also, the data enabled us to monitor the kinetics of the reactions and estimate the rate constants. Conversion of visually interpretable information to color values for quantitative analysis by MIA-TLC is a rapid and inexpensive method recommended as a powerful tool for quantitative reaction monitoring as well as a good alternative for GC in organic laboratories.
Starting Page	933
Ending Page	939
Page Count	7
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	4
Issue Number	4
Journal	Analytical Methods
DOI	10.1039/c2ay25023c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Benzoic acid Benzaldehyde Alkaline hydrolysis Phenyl group Chromatography Digital image processing
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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