NDLI: Separation and quantification of short-chain coenzyme A in plant tissues by capillary electrophoresis with laser-induced fluorescence detection

Content Provider	Royal Society of Chemistry (RSC)
Author	Nikolau, Basil Ma, Yinfa Jiang, Yongqing
Copyright Year	2010
Abstract	Coenzyme A (CoA) is a group of active metabolic compounds that facilitate over 100 chemical reactions in animal and plant cells. It mainly serves as an acyl carrier in many metabolic reactions and initiates the tricarboxylic acid cycle that produces more than 90% of the energy required for life processes. Measurements of short-chain and long-chain CoA compounds in a variety of tissues by using high-performance liquid chromatography (HPLC) and capillary electrophoresis-ultraviolet (CE-UV) detection have been reported, but these techniques do not allow one to simultaneously determine all the possible coexisting CoAs and their derivatives in plant tissues with sufficient sensitivity. In this paper, a method of quantitative determination of 5 short-chain CoAs in plant tissues by using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was developed. Under optimized derivatization and electrophoresis conditions, different CoAs that were derivatized with fluorescein-5-isothiocyanate (FITC) were separated and quantified at the pmole level. A fused silica capillary with a 75 μm (i.d.) × 57 cm was used for the separation and 150 mM borate buffer (pH 9.00) was used as a background electrolyte. The separation was carried out at 25 kV and completed in less than 13 min. The effects of derivatization time, buffer concentrations, and pH values on derivatization efficiency were also systematically investigated. This newly developed CE-LIF method can be used to detect CoAs in both plant and animal tissues.
Starting Page	1900
Ending Page	1904
Page Count	5
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	2
Issue Number	12
Journal	Analytical Methods
DOI	10.1039/c0ay00425a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Laser-induced fluorescence Electrolyte Carboxylic acid Capillary electrophoresis Fluorescein Micrometre Coenzyme A Electrophoresis Isothiocyanate High-performance liquid chromatography Borate Fused quartz
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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