NDLI: Indirect fluorescence of aliphatic carboxylic acids in nonaqueous capillary electrophoresis using merocyanine 540

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Indirect fluorescence of aliphatic carboxylic acids in nonaqueous capillary electrophoresis using merocyanine 540

Content Provider	Scilit
Author	Chiu, Tai-Chia Huang, Ming-Feng Huang, Chih-Ching Hsieh, Ming-Mu Chang, Huan-Tsung
Copyright Year	2002
Description	Journal: Electrophoresis A method for the analysis of aliphatic carboxylic acids (ACAs) in nonaqueous capillary electrophoresis (NACE) in conjunction with indirect laser-induced fluorescence (ILIF) using merocyanine 540 (MC 540) is described. Performing the analysis in organic solvent is advantageous when using MC 540, because of its greater quantum yield in aprotic solvent. To achieve a high dynamic reserve (DR) and optimize resolution, we have tested a number of aqueous mixtures containing alcohols and acetonitrile (ACN). The optimum buffer for the analysis of C2-C18 ACAs, in terms of sensitivity, resolution, and speed, is an aqueous mixture of 40% ACN, 30% ethanol, and 1 mM Tris at apparent pH 7.4 (adjusted with ascorbic acid). Under this condition, the DR is greater than 1000, thereby the limits of detection for acids are in the range of sub-νM to νM. Linear plots show that the dynamic ranges for the analysis of ACAs are at least two decades in concentration, with regression coefficients all greater than 0.98. The relative standard deviations of the migration times and peak heights for all ACAs are less than 2.0%. Furthermore, this simple and cost-effective method has been applied to the analysis of marine lipid concentrate, with the concentrations of 1.67 ± 0.03 and 4.50 plusmn; 0.05 mM (n = 5) for C14 and C16 acids, respectively, in a tablet of marine lipid concentrate sample.
Ending Page	455
Starting Page	449
ISSN	2573508X
e-ISSN	15222683
DOI	10.1002/1522-2683%28200202%2923%3A3%3C449%3A%3Aaid-elps449%3E3.0.co%3B2-p
Journal	Electrophoresis
Issue Number	3
Volume Number	23
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2002-02-18
Access Restriction	Open
Subject Keyword	Journal: Electrophoresis Analytical Chemistry Aliphatic Carboxylic Acid Indirect Laser-induced Fluorescence Merocyanine 540 Nonaqueous Capillary Electrophoresis
Content Type	Text
Resource Type	Article

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