NDLI: A sensitive amperometric immunosensor for hepatitis B surface antigen based on biocompatible redox-active chitosan–toluidine blue/gold nanoparticles composite film

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Analytical Methods Vol - 3 Issue - 5

Analytical Methods Vol - 3 Issue - 4

Analytical Methods Vol - 3 Issue - 3

Analytical Methods Vol - 3 Issue - 2

Analytical Methods Vol -2

Analytical Methods Vol -1

A sensitive amperometric immunosensor for hepatitis B surface antigen based on biocompatible redox-active chitosan–toluidine blue/gold nanoparticles composite film

Content Provider	Royal Society of Chemistry (RSC)
Author	Qiu, Jianding Chen, Yunxia Liang, Ruping
Copyright Year	2011
Abstract	A novel amperometric immunosensor has been developed by self-assembling gold nanoparticles (Au NPs) onto a toluidine blue–branched chitosan (CHIT–TB) modified electrode for the sensitive determination of hepatitis B surface antigen (HBsAg) as a model protein. The formation of CHIT–TB composite film not only effectively avoids the leakage of TB and retains its electrochemical activity, but also enhances the conductivity and charge–transport properties of the composite. Further adsorption of Au NPs into the CHIT matrix provides both the interactive sites for the immobilization of HBsAb and a favorable microenvironment to maintain the activity of the HBsAb, in addition it prevents leakage of HBsAb molecules from the CHIT–TB/Au NPs/HBsAb film structure efficiently. The morphologies and electrochemistry of the formed nanocomposite film were investigated by using scanning electron microscopy and electrochemical techniques including cyclic voltammetry and electrochemical impedance spectroscopy (EIS). The HBsAg concentration was measured through the decrease of amperometric responses in the corresponding specific binding of antigen and antibody. The decreased voltametric values were proportional to the HBsAg concentration in the range of 1 to 200 ng mL−1. The detection of HBsAg levels in five sera obtained from a hospital showed acceptable accuracy.
Starting Page	1338
Ending Page	1343
Page Count	6
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	3
Issue Number	6
Journal	Analytical Methods
DOI	10.1039/c0ay00774a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Composite film Spectroscopy Electrode HBsAg Scanning electron microscope Protein Antigen Cyclic voltammetry Toluidine Adsorption Electrochemistry Extracellular matrix Nanocomposite Chitosan Hepatitis B Electrical impedance
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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