NDLI: More than just heat: ambient ionization mass spectrometry for determination of the species of origin of processed commercial products—application to psychoactive pepper supplements

Content Provider	Royal Society of Chemistry (RSC)
Author	Musah, Rabi A. Lesiak, Ashton D.
Copyright Year	2016
Abstract	The application of direct analysis in real time high resolution mass spectrometry (DART-HRMS) to the determination of the originating species of the plants from which processed commercially available plant-based products are derived is described. As a proof of principle, the method was employed for determination of the provenance of psychoactive pepper species, namely Piper methysticum (aka kava) and P. betle (aka betel). In addition to being of agricultural importance, these species are also of relevance in a forensics context. DART-HRMS spectra showed that extractions, heat treatment and other steps associated with the manufacture of these products result in significant differences in the mass spectral fingerprints observed. Nevertheless, the presence of key species-specific biomarkers such as kavalactones and chalcones in P. methysticum, and a variety of terpenes in P. betle, were retained. Chemometric processing by principal component analysis using a selection of feature masses that represented both compounds common to each of the species, and others that distinguished them, showed that the two Piper spp. could be readily identified, regardless of the manufacturing process used to create the product, with a leave-one-out cross validation result of 100%. Furthermore, unsupervised statistical analysis processing by hierarchical clustering not only enabled P. methysticum and P. betle products to be distinguished from one another, but also permitted further discrimination that was based on the processing method used to produce them. Advantages of the method over commonly used conventional protocols include minimal methods development; the capability of analyzing material in its native form without resorting to solvent extraction, derivatization or other sample preparation steps; speed; and the ability to detect and definitively identify biomarkers characteristic of a species. The method has wide applicability and is particularly useful for analysis of products from plants whose genes have not been mapped, and which, as a consequence, cannot be subjected to DNA analysis to determine the originating plant species.
Starting Page	1646
Ending Page	1658
Page Count	13
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	8
Issue Number	7
Journal	Analytical Methods
DOI	10.1039/c5ay02570b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Chalconoid Cluster analysis Kava Cross-validation (statistics) DNA Psychoactive drug Piper Liquid\u2013liquid extraction Mass spectrometry Areca nut Principal component analysis Piper Aircraft
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Identification and characterization of stress degradation products of piperine and profiling of a black pepper (Piper nigrum L.) extract using LC/Q-TOF-dual ESI-MS

Surface analysis of lipids by mass spectrometry: more than just imaging.

Authentication of processed meat products by peptidomic analysis using rapid ambient mass spectrometry.

Identification of Compounds in Commercial Kava Extracts by Gas Chromatography with Electron Ionization High-Resolution Mass Spectrometry

Coupling neutral desorption sampling to dielectric barrier discharge ionization mass spectrometry for direct oil analysis

Biacore and mass spectrometry : more than just a marriage of convenience

Nontargeted Metabolomics for Phenolic and Polyhydroxy Compounds Profile of Pepper (Piper nigrum L.) Products Based on LC-MS/MS Analysis

Cholesterol Sulfate Imaging in Human Prostate Cancer Tissue by Desorption Electrospray Ionization Mass Spectrometry

Applications of ambient ionization mass spectrometry in 2020: An annual review

More than just heat: ambient ionization mass spectrometry for determination of the species of origin of processed commercial products—application to psychoactive pepper supplements

Similar Documents

Identification and characterization of stress degradation products of piperine and profiling of a black pepper (Piper nigrum L.) extract using LC/Q-TOF-dual ESI-MS

Surface analysis of lipids by mass spectrometry: more than just imaging.

Authentication of processed meat products by peptidomic analysis using rapid ambient mass spectrometry.

Identification of Compounds in Commercial Kava Extracts by Gas Chromatography with Electron Ionization High-Resolution Mass Spectrometry

Coupling neutral desorption sampling to dielectric barrier discharge ionization mass spectrometry for direct oil analysis

Biacore and mass spectrometry : more than just a marriage of convenience

Nontargeted Metabolomics for Phenolic and Polyhydroxy Compounds Profile of Pepper (Piper nigrum L.) Products Based on LC-MS/MS Analysis

Cholesterol Sulfate Imaging in Human Prostate Cancer Tissue by Desorption Electrospray Ionization Mass Spectrometry

Applications of ambient ionization mass spectrometry in 2020: An annual review

More than just heat: ambient ionization mass spectrometry for determination of the species of origin of processed commercial products—application to psychoactive pepper supplements