NDLI: An LC-MS/MS method to determine PABA and DIP

Content Provider	Royal Society of Chemistry (RSC)
Author	Zheng, Mi Xin, Guijie Gu, Jingkai Meng, Xiangjun Yang, Bo Yin, Lei Su, Chong Yang, Zhichao Zhao, Limei Yang, Junfeng
Copyright Year	2016
Abstract	A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method using positive/negative electrospray ionization (ESI) switching for the simultaneous quantitation of p-acetaminobenzoic acid (PABA) and N,N-dimethylamino-2-propanol (DIP) in human plasma has been developed and validated. After simple protein precipitation using acetonitrile, the analytes and internal standard (IS) diazepam were separated on a CAPCELL PAK Phenyl column (150 × 2.0 mm, 5 μm) using formic acid : water (1 : 1000, v/v) as solvent A and acetonitrile as solvent B at a flow rate of 0.4 mL min−1. N,N-Dimethylamino-2-propanol and the IS were detected by positive ion electrospray ionization followed by multiple reaction monitoring (MRM) of the transition at m/z 104.0 → 86.1 and 256.3 → 167.1, respectively. p-Acetaminobenzoic acid was detected by negative ion electrospray ionization followed by MRM of the transitions at m/z 178.1 → 134.0. The method was linear for PABA and DIP in the concentration range of 0.02–10 μg mL−1 with intra- and inter-day precisions (as relative standard deviation) of ≤5.81% and accuracy (as relative error) of ≤6.52% and the limit of detection (LOD) values were 10 and 5 ng mL−1, respectively. The method was successfully applied to a pharmacokinetic study of the analytes in humans after oral administration of 1.0 g inosiplex tablets.
Starting Page	1013
Ending Page	1020
Page Count	8
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	8
Issue Number	5
Journal	Analytical Methods
DOI	10.1039/c5ay02006a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Acid Protein precipitation Electrospray ionization Ion Coefficient of variation Blood plasma Isopropyl alcohol PABA Ionization Acetonitrile 4-Aminobenzoic acid Approximation error ESI Pharmacokinetics Solvent Diazepam
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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