NDLI: <i>In vitro</i> biophysical, microspectroscopic and cytotoxic evaluation of metastatic and non-metastatic cancer cells in responses to anti-cancer drug

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Simultaneous enrichment, separation and detection of mercury(ii) ions using cloud point extraction and colorimetric sensor based on thermoresponsive hyperbranched polymer–gold nanocomposite

In vitro biophysical, microspectroscopic and cytotoxic evaluation of metastatic and non-metastatic cancer cells in responses to anti-cancer drug

A combined technique for the pretreatment of ultra trace bisphenol A in environmental water based on magnetic matrix solid phase extraction assisted dispersive liquid–liquid microextraction

Magnetic ELISA of aflatoxin B1 – pre-concentration without elution

Dendrimer grafted nanoporous silica as a new coating for headspace solid-phase microextraction fibers

Development of an electrochemical method for the determination of bicalutamide at the SWCNT/CPE in pharmaceutical preparations and human biological fluids

An efficient, sensitive and accurate method for the detection of astragaloside IV in Chinese functional spirit by ultra-performance liquid chromatography–quadrupole-orbitrap mass spectrometry

Rapid screening and identification of non-target flavonoid components in invasive weeds by LC/MS-IT-TOF

Ionic electroactive polymer actuators as active microfluidic mixers

Selective and sensitive determination of ochratoxin A based on a molecularly imprinted electrochemical luminescence sensor

An ionic liquid-based microextraction method for highly selective and sensitive trace determination of nickel in environmental and biological samples

Determination and pharmacokinetic study of kaempferide in rat plasma by UPLC

Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplification

Determination of Mo(vi) in water with poly(quaternary ammonium salt) as a binding phase of diffusive gradients in thin-films

Molecularly imprinted polymers prepared using a porogenic solvent of an ionic liquid and a macromolecular crowding agent and their application in purification of oleanic acid

Magnetic nanoparticle solid phase extraction-HPLC-UV for determination of deoxynivalenol in wheat flour

1,8-Naphthyridine-based fluorescent receptors for picric acid detection in aqueous media

Comparative characterization of amino acids in Abelmoschus manihot roots, stems and leaves during different growth periods by UPLC-TQ-MS/MS

Optimization of enzyme immobilization on magnetic microparticles using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) as a crosslinking agent

Dissipation kinetics and residues of amidosulfuron and MCPA in wheat ecosystems based on a modified QuEChERS and low-temperature cleanup method using the RRLC-QqQ-MS/MS technique

Determination of trace Bi by ICP-OES after magnetic solid phase extraction with fullerene C₆₀ modified γ-Fe₂O₃ superparamagnetic iron oxide nanoparticles

Methodologies for the Taylor dispersion analysis for mixtures, aggregates and the mitigation of buffer mismatch effects

Efficient separation of boron using solid-phase extraction for boron isotope analysis by MC-ICP-MS

Characterization and discrimination of saffron by multisensory systems, SPME-GC-MS and UV-Vis spectrophotometry

Electrochemical immunoassay based on polythionine as the signal source for the sensitive detection of carcinoma embryonic antigen

A simple label-free electrochemical method for the detection of polynucleotide kinase activity by a peroxidase mimic: TiO₂ nanotube array

Solid phase extraction of Pb(ii) and Cd(ii) ions based on murexide functionalized magnetic nanoparticles with the aid of experimental design methodology

Large scale preparation of surface enhanced Raman spectroscopy substrates based on silver nanowires for trace chemical detection

A simple colorimetric kit for determination of ketamine hydrochloride in water samples

A fluorescent probe for the discrimination between Cys and GSH

Analysis of latex protein content by liquid chromatography coupled with tandem mass spectrometry (HPLC/MS/MS)

A ‘chromogenic’ and ‘fluorogenic’ bis-Schiff base sensor for rapid detection of hydrazine both in solution and vapour phases

Detection of epinephrine and metanephrine at a nitrogen doped three-dimensional porous graphene modified electrode

Application of electron paramagnetic resonance spectroscopy, Fourier transform infrared spectroscopy-attenuated total reflectance and scanning electron microscopy to the study of the photo-oxidation of wool fiber

Production and characterization of diphenyl ditelluride-loaded nanocapsules: validation using an analytical method

Application of a fluorescently labeled trans-acting antigenomic HDV ribozyme to monitor antibiotic–RNA interactions

A seaweed–polyaniline nanofibre modified electrode for sensing of uric acid

Decoding the signal response of steroids in electrospray ionization mode (ESI-MS)

Application of the Stern–Volmer equation for studying the spectrofluorimetric quenching reaction of eosin with clindamycin hydrochloride in its pure form and pharmaceutical preparations

Isotopic analysis of depleted zinc in Zn-64 using thermal ionization mass spectrometry

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Analytical Methods Vol - 7 Issue - 19

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Analytical Methods Vol - 7 Issue - 8

Analytical Methods Vol - 7 Issue - 7

Analytical Methods Vol - 7 Issue - 6

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Analytical Methods Vol -1

In vitro biophysical, microspectroscopic and cytotoxic evaluation of metastatic and non-metastatic cancer cells in responses to anti-cancer drug

Content Provider	Royal Society of Chemistry (RSC)
Author	Vargis, Elizabeth Li, Qifei Harihar, Sitaram Zhou, Anhong Xiao, Lifu Welch, Danny R.
Copyright Year	2015
Abstract	The Breast Cancer Metastasis Suppressor 1 (BRMS1) is a nucleo-cytoplasmic protein that suppresses cancer metastasis without affecting the growth of the primary tumor. Previous work has shown that it decreases the expression of protein mediators involved in chemoresistance. This study measured the biomechanical and biochemical changes in BRMS1 expression and the responses of BRMS1 to drug treatments on cancer cells in vitro. The results show that BRMS1 expression affects biomechanical properties by decreasing the Young's modulus and adhesion force of breast cancer cells after doxorubicin (DOX) exposure. Raman spectral bands corresponding to DNA/RNA, lipids and proteins were similar for all cells after DOX treatment. The expression of cytokines were similar for cancer cells after DOX exposure, although BRMS1 expression had different effects on the secretion of cytokines for breast cancer cells. The absence of significant changes on apoptosis, reactive oxygen species (ROS) expression and cell viability after BRMS1 expression shows that BRMS1 has little effect on cellular chemoresistance. Analyzing cancer protein expression is critical in evaluating therapeutics. Our study may provide evidence of the benefit of metastatic suppressor expression before chemotherapy.
Starting Page	10162
Ending Page	10169
Page Count	8
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	7
Issue Number	24
Journal	Analytical Methods
DOI	10.1039/c5ay01810b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Reactive oxygen species Chemotherapy BRMS1 RNA DNA Raman Breast cancer Metastasis Doxorubicin Protein Cancer Apoptosis
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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In vitro biophysical, microspectroscopic and cytotoxic evaluation of metastatic and non-metastatic cancer cells in responses to anti-cancer drug.

Low-dose chemotherapeutic drugs induce reactive oxygen species and initiate apoptosis-mediated genomic instability

TP53 alteration determines the combinational cytotoxic effect of doxorubicin and an antioxidant NAC

Linifanib induces apoptosis in human ovarian cancer cells via activation of FOXO3 and reactive oxygen species

Elevated Production of Mitochondrial Reactive Oxygen Species via Hyperthermia Enhanced Cytotoxic Effect of Doxorubicin in Human Breast Cancer Cell Lines MDA-MB-453 and MCF-7

Mitochondrial Breast Cancer Resistant Protein Sustains the Proliferation and Survival of Drug-Resistant Breast Cancer Cells by Regulating Intracellular Reactive Oxygen Species

Mitochondria-Derived Reactive Oxygen Species Play an Important Role in Doxorubicin-Induced Platelet Apoptosis

Iron-Chelated Polydopamine Decorated Doxorubicin-Loaded Nanodevices for Reactive Oxygen Species Enhanced Cancer Combination Therapy

Costunolide enhances doxorubicin-induced apoptosis in prostate cancer cells via activated mitogen-activated protein kinases and generation of reactive oxygen species

In vitro biophysical, microspectroscopic and cytotoxic evaluation of metastatic and non-metastatic cancer cells in responses to anti-cancer drug

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In vitro biophysical, microspectroscopic and cytotoxic evaluation of metastatic and non-metastatic cancer cells in responses to anti-cancer drug.

Low-dose chemotherapeutic drugs induce reactive oxygen species and initiate apoptosis-mediated genomic instability

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Linifanib induces apoptosis in human ovarian cancer cells via activation of FOXO3 and reactive oxygen species

Elevated Production of Mitochondrial Reactive Oxygen Species via Hyperthermia Enhanced Cytotoxic Effect of Doxorubicin in Human Breast Cancer Cell Lines MDA-MB-453 and MCF-7

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In vitro biophysical, microspectroscopic and cytotoxic evaluation of metastatic and non-metastatic cancer cells in responses to anti-cancer drug