NDLI: Rapid identification of H5 avian influenza virus in chicken throat swab specimens using microfluidic real-time RT-PCR

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Back cover

Analytical Methods Vol - 6 Issue - 7

Analytical Methods Vol - 6 Issue - 6

Analytical Methods Vol - 6 Issue - 5

Analytical Methods Vol - 6 Issue - 4

Analytical Methods Vol - 6 Issue - 3

Analytical Methods Vol -5

Analytical Methods Vol -4

Analytical Methods Vol -3

Analytical Methods Vol -2

Analytical Methods Vol -1

Rapid identification of H5 avian influenza virus in chicken throat swab specimens using microfluidic real-time RT-PCR

Content Provider	Royal Society of Chemistry (RSC)
Author	Zhao, Shumi Lin, Jianhan Li, Li Deng, Guoqing Liao, Ming Zhu, Cancan Zhang, Long Jiao, Peirong Liu, Yong Zhu, Ling
Copyright Year	2014
Abstract	Avian influenza virus H5 is a widespread virus among humans and animals which has caused fatally systemic diseases through poultry-to-person transmission in the past few years. Reverse transcription polymerase chain reaction (RT-PCR) has been proved to be an effective approach for the identification and detection of avian influenza viruses. However, conventional tube RT-PCR is slow and reagent consuming and cannot meet the need for rapid and low cost detection of pathogenic bacteria and viruses. Microfluidic PCR is a burgeoning field among the techniques based on molecular analysis. In this paper, we reported a microfluidic PCR system that integrated RT-PCR and real time fluorescence detection for rapid identification of avian influenza virus H5. This microfluidic device mainly consisted of a thermal controlling unit providing actuation for the temperature cycling needed for amplification, an optical inspection system for online recording fluorescence and a microfluidic chip fabricated using polydimethylsiloxane (PDMS). In this study, influenza virus H5 from clinical chicken throat swab specimens was rapidly detected using the RT-PCR microfluidic system, which was consistent with the results of embryonated egg culture. Compared with a large-scale device, the integrated microfluidic system presented here can perform rapid nucleic acid amplification and analysis, possibly making it a crucial platform for pathogenic bacterium and virus detection in the future.
Starting Page	2628
Ending Page	2632
Page Count	5
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	6
Issue Number	8
Journal	Analytical Methods
DOI	10.1039/c3ay42126k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Chain reaction Acid Fluorescence Temperature cycling Reagent Avian influenza Virus Polymerase Polymerase chain reaction Influenza Bacteria Chicken Polydimethylsiloxane Real-time polymerase chain reaction
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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Identification and subtyping of avian influenza viruses by reverse transcription polymerase chain reaction (rt-pcr) and agarose gel electrophoresis.

Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses.

Rapid detection of H5 avian influenza virus by TaqMan-MGB real-time RT-PCR

Typing of Poultry Influenza Virus (H5 and H7) by Reverse Transcription-Polymerase Chain Reaction

Rapid detection of highly pathogenic avian influenza h5n1 virus by taqman reverse transcriptase-polymerase chain reaction.

Removal of Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR) Inhibitors Associated with Cloacal Swab Samples and Tissues for Improved Diagnosis of Avian Influenza Virus by RT-PCR

Typing of Poultry Influenza Virus (H5 and H7) by Reverse Transcription- Polymerase Chain Reaction

Application of real-time reverse transcription polymerase chain reaction to the detection the matrix, H5 and H7 genes of avian influenza viruses in field samples from South Korea

Evaluation of rt-pcr for the detection of influenza virus serotype h9n2 among broiler chickens.

Rapid identification of H5 avian influenza virus in chicken throat swab specimens using microfluidic real-time RT-PCR

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Rapid identification of H5 avian influenza virus in chicken throat swab specimens using microfluidic real-time RT-PCR