NDLI: Quantification of protein secondary structure content by multivariate analysis of deep-ultraviolet resonance Raman and circular dichroism spectroscopies

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Analytical Methods Vol - 6 Issue - 5

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Quantification of protein secondary structure content by multivariate analysis of deep-ultraviolet resonance Raman and circular dichroism spectroscopies

Content Provider	Royal Society of Chemistry (RSC)
Author	Oshokoya, Olayinka O. JiJi, Renee D. Roach, Carol A.
Copyright Year	2014
Abstract	Determination of protein secondary structure (α-helical, β-sheet, and disordered motifs) has become an area of great importance in biochemistry and biophysics as protein secondary structure is directly related to protein function and protein related diseases. While NMR and X-ray crystallography can predict the placement of each atom in a protein to within an angstrom, optical methods (i.e. CD, Raman, and IR) are the preferred techniques for rapid evaluation of protein secondary structure content. Such techniques require calibration data to predict unknown protein secondary structure content where accuracy may be improved with the application of multivariate analysis. Here, a comparison of the protein secondary structure predictions obtained from multivariate analysis of ultraviolet resonance Raman (UVRR) and circular dichroism (CD) spectroscopic data using classical least squares (CLS), partial least squares (PLS), and multivariate curve resolution-alternating least squares (MCR-ALS) is made. Results of the multivariate analysis suggest that CD measurements provide more accurate prediction of protein α-helical content whereas UVRR more accurately predicts β-sheet content, an observation that is consistent with previous studies. Based on this analysis, it is suggested that the best approach to rapid and accurate protein secondary structure determination is to combine both CD and UVRR spectroscopic data.
Starting Page	1691
Ending Page	1699
Page Count	9
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	6
Issue Number	6
Journal	Analytical Methods
DOI	10.1039/c3ay42032a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Ångström Biochemistry Biophysics Multivariate analysis Ultraviolet Biomolecular structure Protein Circular dichroism X-ray crystallography Classical mechanics Raman Beta sheet Nuclear magnetic resonance Resonance Raman spectroscopy
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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