NDLI: A sensitive heterogeneous biotin–streptavidin enzyme-linked immunosorbent assay for the determination of di-(2-ethylhexyl)phthalate (DEHP) in beverages using a specific polyclonal antibody

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A sensitive heterogeneous biotin–streptavidin enzyme-linked immunosorbent assay for the determination of di-(2-ethylhexyl)phthalate (DEHP) in beverages using a specific polyclonal antibody

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A sensitive heterogeneous biotin–streptavidin enzyme-linked immunosorbent assay for the determination of di-(2-ethylhexyl)phthalate (DEHP) in beverages using a specific polyclonal antibody

Content Provider	Royal Society of Chemistry (RSC)
Author	Zhuang, Huisheng Sun, Ruiyan
Copyright Year	2014
Abstract	Di-(2-ethylhexyl)phthalate (DEHP) is one of the long-chain or high-molecular-weight compounds of the phthalic acid diester (PAE) family, which is the most commonly used plasticizer and additive. However, DEHP may cause birth defects, sexual dysfunction, even cancers, possibly heart disease, etc. In order to detect DEHP with high sensitivity and specificity, an indirect competitive biotin–streptavidin enzyme-linked immunosorbent assay (BA-ELISA) has been established in this study. A specific polyclonal antibody (pAb-DEHP) targeting DEHP was obtained first, and the procedures of BA-ELISA were optimized for the determination of DEHP in beverages. Under optimal conditions, a good linearity was achieved within a range of 0.021 to 12.948 μg L−1. The limit of detection (IC10) was 0.0074 μg L−1 and the median inhibitory concentration (IC50) was 0.526 μg L−1. The BA-ELISA was highly selective, with low cross-reactivity values with DEHP analogues (lower than 7%). Finally, the assay was successfully used to detect DEHP in beverages; the concentrations of DEHP in the samples ranged from 1.18 μg L−1 to 40.68 μg L−1. Satisfactory recoveries (89.07–109.33%) and coefficient of variation (CV) values (5.97% to 10.68%) were obtained, which further confirmed that this proposed BA-ELISA immunoassay is sensitive, rapid and accurate for monitoring DEHP in the environment.
Starting Page	9807
Ending Page	9815
Page Count	9
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	6
Issue Number	24
Journal	Analytical Methods
DOI	10.1039/c4ay02088j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Ester Immunoassay Phthalic acid Plasticizer Carboxylic acid Sexual dysfunction IC50 Bis(2-ethylhexyl) phthalate Polyclonal antibodies ELISA
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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Biotin-Streptavidin Enzyme-linked Immunosorbent Assay for the Determination of Dibutyl Phthalate in Beverages and Drinking Water Using a Specific Polyclonal Antibody

IUPAC Systematic Name: Bis(2-ethylhexyl)

Synthesis of an artificial antigen and preparation of a polyclonal antibody for the sensitive determination of phthalate esters by enzyme-linked immunoassay

Development of enzyme linked immunosorbent assay for erythropoietin.

by Capsular Polysaccharide-Biotin-Streptavidin Enzyme-Linked Immunosorbent Assay (2000)

Toxicology of di-2-ethylhexyl phthalate and other phthalic acid ester plasticizers.

Renal function profile of Di (2-Ethylhexyl) phthalate exposed adult Wistar Rats

The In Vitro Serum Protein-Binding Characteristics of Bis-(2-Ethylhexyl) Phthalate and Its Principal Metabolite, Mono-(2-Ethylhexyl) Phthalate

A Magnetic Nanoparticle Based Enzyme-Linked Immunosorbent Assay for Sensitive Quantification of Zearalenone in Cereal and Feed Samples

A sensitive heterogeneous biotin–streptavidin enzyme-linked immunosorbent assay for the determination of di-(2-ethylhexyl)phthalate (DEHP) in beverages using a specific polyclonal antibody

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Biotin-Streptavidin Enzyme-linked Immunosorbent Assay for the Determination of Dibutyl Phthalate in Beverages and Drinking Water Using a Specific Polyclonal Antibody

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A sensitive heterogeneous biotin–streptavidin enzyme-linked immunosorbent assay for the determination of di-(2-ethylhexyl)phthalate (DEHP) in beverages using a specific polyclonal antibody