NDLI: Non-invasive detection of biomechanical and biochemical responses of human lung cells to short time chemotherapy exposure using AFM and confocal Raman spectroscopy

Please wait, while we are loading the content...

Non-invasive detection of biomechanical and biochemical responses of human lung cells to short time chemotherapy exposure using AFM and confocal Raman spectroscopy

Novel pH sensor based on anthraquinone–ferrocene modified free standing gold nanowire array electrode

A novel detection of nitrite, iodate and bromate based on a luminescent polyoxometalate

Determination of the empirical solvent polarity parameter E_T(30) by multivariate image analysis

Development and validation of analytical method for determination of total urinary antimony by chelation in supercritical carbon dioxide using fluorinated chelating agents

Eliminating the matrix effects of dissolved carbon dioxide and carbonate in extraction of triclosan and chlorophenols in environmental waters by hollow fiber supported liquid membrane

Improved sensing performance from methionine capped CdTe and CdTe/ZnS quantum dots for the detection of trace amounts of explosive chemicals in liquid media

Visual detection of glyphosate in environmental water samples using cysteamine-stabilized gold nanoparticles as colorimetric probe

A sensitive and specific enzyme immunoassay for detecting tartrazine in human urinary samples

Development of an electroanalytical sensor for γ-hexachlorocyclohexane based on a cellulose acetate modified glassy carbon electrode

Preparation of a novel skeleton weak anion-exchange monolith by single electron transfer-living radical polymerization for protein separation in high performance liquid chromatography

Label-free detection of the foodborne pathogens of Enterobacteriaceae by surface-enhanced Raman spectroscopy

Toxic effects of Euphorbia pekinensis Rupr. and development of a validated UPLC/MS/MS method for profiling of urine metabolic changes

Detection of 6-benzylaminopurine plant growth regulator in bean sprouts using OFRR biosensor and QuEChERS method

Liquid chromatography tandem mass spectrometry method (LC–MS/MS) for simultaneous determination of piperine, cinnamic acid and gallic acid in rat plasma using a polarity switch technique

Rapid monitoring and assessment of pollutional load in dairy waste water

A novel method of chemiluminescence detection coupled with high-performance liquid chromatography and its application in direct determination of tartaric, malic and citric acids in fruit juice

Ultra-trace determination of Hg(ii) in drinking water and local Thai liquors using homogeneous liquid–liquid extraction followed by fluorescence quenching of its ternary complex

Determination of the mineral composition of Brazilian rice and evaluation using chemometric techniques

Determination of estrogens in animal-derived food by matrix solid-phase dispersion extraction coupled with ultra performance liquid chromatography-quadruple time of flight mass spectrometry

Polypyrrole nanotubes–polyaniline composite for DNA detection using methylene blue as intercalator

Development of an HPLC–MS/MS method for the simultaneous analysis of six kinds of parabens in food

One step electrochemical synthesis of gold-nanoparticles–polypyrrole composite for application in catechin electrochemical biosensor

Optimization of ionic liquid-based microwave-assisted dispersive liquid–liquid microextraction for the determination of plasticizers in water by response surface methodology

Using a flow-batch analyzer for photometric determination of Fe(iii) in edible and lubricating oils without external pretreatment

Spectrophotometric determination of phenol by flow injection on-line preconcentration with a micro-column containing magnetic microspheres functionalized with Cyanex272

Simultaneous determination of thiodicarb and its main metabolite residues in cotton by ultra-performance liquid chromatography coupled to tandem mass spectrometry

Combining enzymatic ¹⁸O-labeling and 2-D LC-MS/MS for a study of protein interactions in primary T cells

A simple and specific quantitative method for determination of dictamnine in Dictamni Cortex by ¹H NMR spectroscopy

Ion selective electrodes are not suitable for measurements of silver ion concentrations in alkaline carbonate media

Back cover

Analytical Methods Vol - 5 Issue - 3

Analytical Methods Vol -4

Analytical Methods Vol -3

Analytical Methods Vol -2

Analytical Methods Vol -1

Non-invasive detection of biomechanical and biochemical responses of human lung cells to short time chemotherapy exposure using AFM and confocal Raman spectroscopy

Content Provider	Royal Society of Chemistry (RSC)
Author	Li, Qifei Tang, Mingjie Zhou, Anhong Xiao, Lifu
Copyright Year	2013
Abstract	Cellular biomechanical properties including cell elasticity and cell adhesion are regarded as criteria to differentiate cancer cells and normal cells. In this study, the biomechanical properties including the Young's modulus and adhesion force of human lung adenocarcinoma epithelial cell line A549 and non-cancerous human primary small airway epithelial cells (SAECs) were measured by using atomic force microscopy (AFM). It was found that primary SAECs are stiffer and more adhesive than cancerous A549 cells. Upon treatment with anti-cancer drug doxorubicin (DOX) for a short time (4 hours), both biomechanical properties of A549 cells were found to be increased while those of SAECs were decreased, implying that DOX-induced response mechanisms are different between these two types of cells (cancerous vs. primary cells). Using confocal Raman spectroscopy, we measured the changes in (sub)cellular biochemical compositions of both cell types before and after DOX exposure. Our ultimate goal is to find out the potential relationship between the changes in biomechanics and biochemical compositions of lung epithelial cells in response to anti-cancer drugs.
Starting Page	874
Ending Page	879
Page Count	6
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	5
Issue Number	4
Journal	Analytical Methods
DOI	10.1039/c2ay25951f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Adenocarcinoma Biomechanics Short Time Atomic-force microscopy Cell adhesion Raman Epithelium Raman spectroscopy Doxorubicin Cancer
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Non-invasive detection of biomechanical and biochemical responses of human lung cells to short time chemotherapy exposure using AFM and confocal Raman spectroscopy

Monitoring doxorubicin cellular uptake and trafficking using in vitro Raman microspectroscopy: short and long time exposure effects on lung cancer cell lines

Evolution of the graphite surface in phosphoric acid: an AFM and Raman study

Characterization of nanofibers for tissue engineering: Chemical mapping by Confocal Raman microscopy

Chondrocyte cell adhesion on chitosan supports using single-cell atomic force microscopy

A Semi-Automated Positioning System for Contact-Mode Atomic Force Microscopy (AFM)

Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes

Nanobiosensors Based on Chemically Modified AFM Probes: A Useful Tool for Metsulfuron-Methyl Detection

Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy

Non-invasive detection of biomechanical and biochemical responses of human lung cells to short time chemotherapy exposure using AFM and confocal Raman spectroscopy

Similar Documents

Non-invasive detection of biomechanical and biochemical responses of human lung cells to short time chemotherapy exposure using AFM and confocal Raman spectroscopy

Monitoring doxorubicin cellular uptake and trafficking using in vitro Raman microspectroscopy: short and long time exposure effects on lung cancer cell lines

Evolution of the graphite surface in phosphoric acid: an AFM and Raman study

Characterization of nanofibers for tissue engineering: Chemical mapping by Confocal Raman microscopy

Chondrocyte cell adhesion on chitosan supports using single-cell atomic force microscopy

A Semi-Automated Positioning System for Contact-Mode Atomic Force Microscopy (AFM)

Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes

Nanobiosensors Based on Chemically Modified AFM Probes: A Useful Tool for Metsulfuron-Methyl Detection

Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy

Non-invasive detection of biomechanical and biochemical responses of human lung cells to short time chemotherapy exposure using AFM and confocal Raman spectroscopy