NDLI: High-throughput suspension array for detecting four pathogens

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High-throughput suspension array for detecting four pathogens

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Back cover

Analytical Methods Vol - 4 Issue - 7

Analytical Methods Vol - 4 Issue - 6

Analytical Methods Vol - 4 Issue - 5

Analytical Methods Vol - 4 Issue - 4

Analytical Methods Vol - 4 Issue - 3

Analytical Methods Vol - 4 Issue - 2

Analytical Methods Vol -3

Analytical Methods Vol -2

Analytical Methods Vol -1

High-throughput suspension array for detecting four pathogens

Content Provider	Royal Society of Chemistry (RSC)
Author	Peng, Yuan Sun, Zhiyong Gao, Zhixian Chen, Huishuang Su, Pu Liu, Jianqing Ning, Baoan Bai, Jialei
Copyright Year	2012
Abstract	A new suspension array technology is proposed for the simultaneous quantitative determination of four pathogens in food. Four sets of primers and species-specific capture probes were designed based on the 16S rDNA gene sequences of Escherichia coli, Staphylococcus aureus, Vibrio parahaemolyticus, and Salmonella downloaded from GenBank. The specific nucleic acid probes were covalently bound to the surface of fluorescent microspheres for liquid suspension hybridization. The biotin-labeled PCR products obtained from the samples were hybridized with their complementary nucleic acid probes, which were detected after the addition of streptavidin phycoerythrin. No cross-reaction occurred among the products, and the sensitivity of the probe in the single-channel method was 5 × 10−6 mM. In the multi-channel method, the sensitivities were as follows: 5 × 10−4 mM for E. coli and Salmonella, and 5 × 10−5 mM for S. aureus and V. parahaemolyticus. The multi-channel method achieved multidetection of several pathogens. For real sample detection, the methods showed a sensitivity of 103 CFU per g to 100 CFU per g of vegetables after enrichment for 24 h at 37 °C. This study demonstrates the utility of the suspension array specific for the 16S rDNA gene for determining the presence of the four pathogens in food samples.
Starting Page	2528
Ending Page	2536
Page Count	9
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	4
Issue Number	8
Journal	Analytical Methods
DOI	10.1039/c2ay25327e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Polymerase chain reaction Salmonella Acid Aureus 16S ribosomal RNA Escherichia coli Vibrio cholerae Streptavidin Phycoerythrin Vibrio parahaemolyticus GenBank DNA sequencing
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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