NDLI: Development of a chemiluminescent enzyme immunoassay for the determination of dexamethasone in milk

Content Provider	Royal Society of Chemistry (RSC)
Author	Gribas, Anastasia V. Vdovenko, Marina M. Vylegzhanina, Alexandra V. Sakharov, Ivan Yu.
Copyright Year	2012
Abstract	An indirect competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for the determination of dexamethasone (DEX) was developed using soybean peroxidase (SbP) as an enzyme label. A mixture of 3-(10′-phenothiazinyl)-propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORPH) was used as an enhancer of SbP-induced chemiluminescence. Varying the concentrations of the capture antigen (DEX-ovalbumin) and specific anti-DEX antibody, the conditions of the assay were optimized. The values of IC10, IC50 and working range (IC20–IC80) of the CL-ELISA of DEX were 0.02, 0.9, 0.08–9.3 ng mL−1, respectively. It was shown that a pretreatment of cow milk samples by centrifugation and 25% methanol prevented the matrix effect of whole milk. The coefficient of variation (CV) and recovery values from the spiked milk samples estimated by the developed CL-ELISA were in the range of 2.2 to 9.9% and 82 to 142%, respectively.
Starting Page	2550
Ending Page	2554
Page Count	5
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	4
Issue Number	8
Journal	Analytical Methods
DOI	10.1039/c2ay25278c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Dexamethasone Enzyme Centrifugation Chemiluminescence Soybean Antigen DEX IC50 Positive-definite matrix Peroxidase Propane ELISA Methanol
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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