NDLI: A Major Secreted Elastase Is Essential for Pathogenicity of Aeromonas hydrophila

Content Provider	PubMed Central
Author	Cascón, Alberto Yugueros, Javier Temprano, Alejandro Sánchez, María Hernanz, Carmen Luengo, José María Naharro, Germán
Editor	Burns, D. L.
Copyright Year	2000
Abstract	Aeromonas hydrophila is an opportunistic pathogen and the leading cause of fatal hemorrhagic septicemia in rainbow trout. A gene encoding an elastolytic activity, ahyB, was cloned from Aeromonas hydrophila AG2 into pUC18 and expressed in Escherichia coli and in the nonproteolytic species Aeromonas salmonicida subsp. masoucida. Nucleotide sequence analysis of the ahyB gene revealed an open reading frame of 1,764 nucleotides with coding capacity for a 588-amino-acid protein with a molecular weight of 62,728. The first 13 N-terminal amino acids of the purified protease completely match those deduced from DNA sequence starting at AAG (Lys-184). This finding indicated that AhyB is synthesized as a preproprotein with a 19-amino-acid signal peptide, a 164-amino-acid N-terminal propeptide, and a 405-amino-acid intermediate which is further processed into a mature protease and a C-terminal propeptide. The protease hydrolyzed casein and elastin and showed a high sequence similarity to other metalloproteases, especially with the mature form of the Pseudomonas aeruginosa elastase (52% identity), Helicobacter pylori zinc metalloprotease (61% identity), or proteases from several species of Vibrio (52 to 53% identity). The gene ahyB was insertionally inactivated, and the construct was used to create an isogenic ahyB mutant of A. hydrophila. These first reports of a defined mutation in an extracellular protease of A. hydrophila demonstrate an important role in pathogenesis.
Starting Page	3233
File Format	PDF
ISSN	10985522
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	6
Volume Number	68
Language	English
Publisher	American Society for Microbiology
Publisher Date	2000-06-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The UDP N-Acetylgalactosamine 4-Epimerase Gene Is Essential for Mesophilic Aeromonas hydrophila Serotype O34 Virulence

A Type III Secretion System Is Required for Aeromonas hydrophila AH-1 Pathogenesis

Defined Deletion Mutants Demonstrate that the Major Secreted Toxins Are Not Essential for the Virulence of Aeromonas salmonicida

The Cytotoxic Enterotoxin of Aeromonas hydrophila Is Aerolysin

DNA Adenine Methyltransferase Influences the Virulence of Aeromonas hydrophila

Aeromonas hydrophila virulence

Cytotoxic activity of Aeromonas hydrophila.

Contribution of nuclease to the pathogenesis of Aeromonas hydrophila

Microarray Analysis of Aeromonas hydrophila Cytotoxic Enterotoxin-Treated Murine Primary Macrophages

A Major Secreted Elastase Is Essential for Pathogenicity of Aeromonas hydrophila

Similar Documents

The UDP N-Acetylgalactosamine 4-Epimerase Gene Is Essential for Mesophilic Aeromonas hydrophila Serotype O34 Virulence

A Type III Secretion System Is Required for Aeromonas hydrophila AH-1 Pathogenesis

Defined Deletion Mutants Demonstrate that the Major Secreted Toxins Are Not Essential for the Virulence of Aeromonas salmonicida

The Cytotoxic Enterotoxin of Aeromonas hydrophila Is Aerolysin

DNA Adenine Methyltransferase Influences the Virulence of Aeromonas hydrophila

Aeromonas hydrophila virulence

Cytotoxic activity of Aeromonas hydrophila.

Contribution of nuclease to the pathogenesis of Aeromonas hydrophila

Microarray Analysis of Aeromonas hydrophila Cytotoxic Enterotoxin-Treated Murine Primary Macrophages

A Major Secreted Elastase Is Essential for Pathogenicity of Aeromonas hydrophila