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| Content Provider | PubMed Central |
|---|---|
| Author | Watson, Robert J. Heys, Roselyn Martin, Teresa Savard, Marc |
| Copyright Year | 2001 |
| Abstract | Sinorhizobium meliloti is usually cultured in rich media containing yeast extract. It has been suggested that some components of yeast extract are also required for growth in minimal medium. We tested 27 strains of this bacterium and found that none were able to grow in minimal medium when methods to limit carryover of yeast extract were used during inoculation. By fractionation of yeast extract, two required growth factors were identified. Biotin was found to be absolutely required for growth, whereas previously the need for this vitamin was considered to be strain specific. All strains also required supplementation with cobalt or methionine, consistent with the requirement for a vitamin B12-dependent homocysteine methyltransferase for methionine biosynthesis. |
| Related Links | http://dx.doi.org/10.1128/AEM.67.8.3767-3770.2001 |
| Starting Page | 3767 |
| File Format | |
| ISSN | 10985336 |
| e-ISSN | 10985336 |
| Journal | Applied and Environmental Microbiology |
| Issue Number | 8 |
| Volume Number | 67 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher Date | 2001-08-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ecology Food Science Applied Microbiology and Biotechnology Biotechnology |
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