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| Content Provider | PubMed Central |
|---|---|
| Author | Bergès, Hélène Lauber, Emmanuelle Liebe, Carine Batut, Jacques Kahn, Daniel Bruijn, Frans J. De Frédéric, Ampe |
| Copyright Year | 2003 |
| Abstract | In order to prepare for whole-genome expression analysis in Sinorhizobium meliloti, pilot DNA macroarrays were designed for 34 genes of known regulation. The experimental parameters assessed were the length of the PCR products, the influence of a tag at the 5′ end of the primers, and the method of RNA labeling. Variance and principal-component analysis showed that the most important nonbiological parameter was the labeling method. The sizes of PCR products were also found to be important, whereas the influence of 5′ tags was minimal. The variability between replicated spots on a membrane was found to be low. These experimental procedures were validated by analyzing the effects of microaerobic conditions on gene expression. |
| Related Links | http://dx.doi.org/10.1128/AEM.69.2.1214-1219.2003 |
| Starting Page | 1214 |
| File Format | |
| ISSN | 10985336 |
| e-ISSN | 10985336 |
| Journal | Applied and Environmental Microbiology |
| Issue Number | 2 |
| Volume Number | 69 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher Date | 2003-02-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ecology Food Science Applied Microbiology and Biotechnology Biotechnology |
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