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| Content Provider | PubMed Central |
|---|---|
| Author | Kremer, Kristin Au, Betty Kam Yan Yip, Peter Chi Wai Robin, Skuce Philip, Supply Kam, Kai Man Van, Soolingen Dick |
| Copyright Year | 2005 |
| Abstract | The Mycobacterium tuberculosis Beijing family isolates may cause more than a quarter of all tuberculosis cases worldwide, are emerging in some areas, and are often associated with drug resistance. Early recognition of transmission of this genotype is therefore important. To evaluate the usefulness of variable-number tandem-repeat (VNTR) typing to discriminate and recognize strains of the Beijing family, M. tuberculosis isolates from Hong Kong were subjected to VNTR analysis, spoligotyping, and IS6110 restriction fragment length polymorphism (RFLP) typing. The allelic diversity of the 14 VNTR loci included in the analysis varied from 0 to 0.618 among Beijing strains. The discriminatory power of VNTR analysis was slightly lower than that of IS6110 RFLP. Our analysis shows that VNTR typing, which has many practical advantages over RFLP typing, can be used for epidemiological studies of Beijing strains. However, VNTR-defined clusters should be subtyped with IS6110 RFLP for maximal resolution. |
| Related Links | http://dx.doi.org/10.1128/JCM.43.1.314-320.2005 |
| Starting Page | 314 |
| File Format | |
| ISSN | 1098660X |
| e-ISSN | 1098660X |
| Journal | Journal of Clinical Microbiology |
| Issue Number | 1 |
| Volume Number | 43 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher Date | 2005-01-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology (medical) |
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