NDLI: Phasing the conformational unit of spectrin.

Please wait, while we are loading the content...

Year: 2016, Volume: 113

Year: 2015, Volume: 112

Year: 2014, Volume: 111

Year: 2013, Volume: 110

Year: 2012, Volume: 109

Year: 2011, Volume: 109

Year: 2011, Volume: 108

Year: 2010, Volume: 107

Year: 2009, Volume: 106

Year: 2009, Volume: 94

Year: 2008, Volume: 105

Year: 2007, Volume: 104

Year: 2006, Volume: 103

Year: 2005, Volume: 103

Year: 2005, Volume: 102

Year: 2004, Volume: 101

Year: 2003, Volume: 100

Year: 2002, Volume: 99

Year: 2001, Volume: 98

Year: 2000, Volume: 97

Year: 1999, Volume: 96

Year: 1998, Volume: 95

Year: 1997, Volume: 94

Year: 1996, Volume: 93

Year: 1995, Volume: 92

Year: 1994, Volume: 91

Year: 1993, Volume: 90

Year: 1992, Volume: 89

Year: 1991, Volume: 88

Year: 1991, Volume: 88, Issue: 24

Year: 1991, Volume: 88, Issue: 23

Possible involvement of (2'5')oligoadenylate synthetase activity in pre-mRNA splicing.

Three-dimensional structure of the Escherichia coli phosphocarrier protein IIIglc.

Expression and assembly of spectrally active recombinant holophytochrome.

In vitro assembly of apophytochrome and apophytochrome deletion mutants expressed in yeast with phycocyanobilin.

DNA binding by the archaeal histone HMf results in positive supercoiling.

Disulfide cross-linking studies of the transmembrane regions of the aspartate sensory receptor of Escherichia coli.

Uptake of N-(4'-pyridoxyl)amines and release of amines by renal cells: a model for transporter-enhanced delivery of bioactive compounds.

Two additional protein-tyrosine kinases expressed in human lung: fourth member of the fibroblast growth factor receptor family and an intracellular protein-tyrosine kinase.

A dominant positive and negative selectable gene for use in mammalian cells.

A steroid-inducible gene expression system for plant cells.

virF, the host-range-determining virulence gene of Agrobacterium tumefaciens, affects T-DNA transfer to Zea mays.

t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene, AML1.

Elevated content of the tyrosine kinase substrate phospholipase C-gamma 1 in primary human breast carcinomas.

The pharmacokinetics of, and humoral responses to, antigen delivered by microencapsulated liposomes.

HLA class I-restricted human cytotoxic T cells recognize endogenously synthesized hepatitis B virus nucleocapsid antigen.

A triplex DNA-binding protein from human cells: purification and characterization.

Activating and inactivating mutations of the alpha subunit of Gi2 protein have opposite effects on proliferation of NIH 3T3 cells.

Masculinization of female isopods (Crustacea) correlated with non-Mendelian inheritance of cytoplasmic viruses.

DNA hybridization evidence for the Australasian affinity of the American marsupial Dromiciops australis.

Characterization of the interaction of plant transcription factors using a bacterial repressor protein.

Heat shock proteins can regulate expression of the Tla region-encoded class Ib molecule Qa-1.

Purification and characterization of particulate endothelium-derived relaxing factor synthase from cultured and native bovine aortic endothelial cells.

Proteolysis patterns of epitopically labeled yeast DNA topoisomerase II suggest an allosteric transition in the enzyme induced by ATP binding.

Rapid dissociation and reassociation of actomyosin cross-bridges during force generation: a newly observed facet of cross-bridge action in muscle.

Efficient detection of three-dimensional structural motifs in biological macromolecules by computer vision techniques.

p55CDC25 is a nuclear protein required for the initiation of mitosis in human cells.

Expression of Rous sarcoma virus-derived retroviral vectors in the avian blastoderm: potential as stable genetic markers.

GAL4 is phosphorylated as a consequence of transcriptional activation.

Interplay of two cis-acting mRNA regions in translational control of sigma 32 synthesis during the heat shock response of Escherichia coli.

Photosensitivity of a protoporphyrin-accumulating, light-sensitive mutant (visA) of Escherichia coli K-12.

The promoter of the CD11b gene directs myeloid-specific and developmentally regulated expression.

T lymphocytes inhibit the vascular response to injury.

Protection against endotoxic shock by a tumor necrosis factor receptor immunoadhesin.

Excess brain protein oxidation and enzyme dysfunction in normal aging and in Alzheimer disease.

Identification of the second common Jewish Gaucher disease mutation makes possible population-based screening for the heterozygous state.

Phosphorylation-dependent regulation of axon fasciculation.

Functional analysis of an oxygen-regulated transcriptional enhancer lying 3' to the mouse erythropoietin gene.

Gene transfer with subsequent removal of the selection gene from the host genome.

Phosphorylation controls binding of acidic proteins to the ribosome.

A 70-amino acid zinc-binding polypeptide from the regulatory chain of aspartate transcarbamoylase forms a stable complex with the catalytic subunit leading to markedly altered enzyme activity.

Evaluating the effects of a single amino acid substitution on both the native and denatured states of a protein.

Transfer of oxygen from an artificial protease to peptide carbon during proteolysis.

Mechanism of cyanide inhibition of the blood-clotting, vitamin K-dependent carboxylase.

Immunoglobulin-like PapD chaperone caps and uncaps interactive surfaces of nascently translocated pilus subunits.

Ribozyme-mediated cleavage of c-fos mRNA reduces gene expression of DNA synthesis enzymes and metallothionein.

Nucleosome positioning is determined by the (H3-H4)2 tetramer.

Intramolecular crosslinking of monomeric fibrinogen by tissue transglutaminase.

Ring fission of anthracene by a eukaryote.

Introduction of disease-related mitochondrial DNA deletions into HeLa cells lacking mitochondrial DNA results in mitochondrial dysfunction.

Surface expression of alternative forms of the TCR/CD3 complex.

Frequency and severity of cyclic flow alternations and platelet aggregation predict the severity of neointimal proliferation following experimental coronary stenosis and endothelial injury.

Functional expression cloning and characterization of the hepatocyte Na+/bile acid cotransport system.

Chronic lithium regulates the expression of adenylate cyclase and Gi-protein alpha subunit in rat cerebral cortex.

Activation of the erythropoietin receptor promoter by transcription factor GATA-1.

Expression of GATA-binding proteins during embryonic development in Xenopus laevis.

Evidence for common ancestry of a chestnut blight hypovirulence-associated double-stranded RNA and a group of positive-strand RNA plant viruses.

Role of transfection and clonal selection in mediating radioresistance.

Mutations in p53 as potential molecular markers for human breast cancer.

CD10/neutral endopeptidase 24.11 hydrolyzes bombesin-like peptides and regulates the growth of small cell carcinomas of the lung.

Reactions of the subunits of the class II major histocompatibility complex molecule IAd.

Identification of negative-strand complements to cytochrome oxidase subunit III RNA in Trypanosoma brucei.

Characterization of a mammalian smooth muscle myosin heavy-chain gene: complete nucleotide and protein coding sequence and analysis of the 5' end of the gene.

Characterization of human mineralocorticosteroid receptor expressed in the baculovirus system.

A contingent replication assay for the detection of protein-protein interactions in animal cells.

Selective binding of activated pp60c-src by an immobilized synthetic phosphopeptide modeled on the carboxyl terminus of pp60c-src.

Involvement of gap junctions in tumorigenesis: transfection of tumor cells with connexin 32 cDNA retards growth in vivo.

Identification of 10 murine homeobox genes.

Detection of homeobox genes in development and evolution.

Functional analysis in yeast of cDNA coding for the mitochondrial Rieske iron-sulfur protein of higher plants.

Mouse kappa light-chain recombination signal sequences mediate recombination more frequently than do those of lambda light chain.

A broadly neutralizing monoclonal antibody that recognizes the V3 region of human immunodeficiency virus type 1 glycoprotein gp120.

Toward an animal model of cystic fibrosis: targeted interruption of exon 10 of the cystic fibrosis transmembrane regulator gene in embryonic stem cells.

Identification of a gene, MLL, that spans the breakpoint in 11q23 translocations associated with human leukemias.

Inhibition of the activation of Hageman factor (factor XII) by human vascular endothelial cell culture supernates.

Immunizations of monkeys with synthetic peptides disclose conserved areas on gp120 of human immunodeficiency virus type 1 associated with cross-neutralizing antibodies and T-cell recognition.

A mutation in a Rhodobacter capsulatus gene encoding an integration host factor-like protein impairs in vivo hydrogenase expression.

Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells.

(-)-baclofen and gamma-aminobutyric acid inhibit calcium currents in isolated retinal ganglion cells.

Immunological identification and characterization of a delayed rectifier K+ channel polypeptide in rat brain.

Dihydropyridine receptor of L-type Ca2+ channels: identification of binding domains for [3H](+)-PN200-110 and [3H]azidopine within the alpha 1 subunit.

Beta 3-adrenergic receptor stimulation restores message and expression of brown-fat mitochondrial uncoupling protein in adult dogs.

The plastid genome of Cryptomonas phi encodes an hsp70-like protein, a histone-like protein, and an acyl carrier protein.

Phasing the conformational unit of spectrin.

A herpesvirus maturational proteinase, assemblin: identification of its gene, putative active site domain, and cleavage site.

A plausible model for reversal of neoplastic transformations in plants based on multiple steady states.

Structural polymorphism in the major groove of a 5S RNA gene complements the zinc finger domains of transcription factor IIIA.

Phytochrome a overexpression inhibits hypocotyl elongation in transgenic Arabidopsis.

Structure of the human villin gene.

Protein kinase C activates an H+ (equivalent) conductance in the plasma membrane of human neutrophils.

An extraovarian protein accumulated in mosquito oocytes is a carboxypeptidase activated in embryos.

Antiviral activity of 1-docosanol, an inhibitor of lipid-enveloped viruses including herpes simplex.

Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide.

Identification of cyclic AMP as the response regulator for neurosecretory potentiation: a memory model system.

Homologous recombination catalyzed by a nuclear extract from Xenopus oocytes.

Efficient transformation of cam2, a behavioral mutant of Paramecium tetraurelia, with the calmodulin gene.

Abnormal expression of two microtubule-associated proteins (MAP2 and MAP5) in specific subfields of the hippocampal formation in schizophrenia.

Formation of cage-like particles by poly(amino acid)-based block copolymers in aqueous solution.

Reversible conversion of nitroxyl anion to nitric oxide by superoxide dismutase.

URF13, a maize mitochondrial pore-forming protein, is oligomeric and has a mixed orientation in Escherichia coli plasma membranes.

DNA looping between the origin of replication of Epstein-Barr virus and its enhancer site: stabilization of an origin complex with Epstein-Barr nuclear antigen 1.

Epstein-Barr nuclear antigen 1 mediates a DNA loop within the latent replication origin of Epstein-Barr virus.

Contribution of the hydrophobic effect to protein stability: analysis based on simulations of the Ile-96----Ala mutation in barnase.

Deletion of the c-kit protooncogene in the human developmental defect piebald trait.

Lymphocytes with a CD4+ CD8- CD3- phenotype are effectors of experimental cutaneous graft-versus-host disease.

Crystals of a ternary complex of human immunodeficiency virus type 1 reverse transcriptase with a monoclonal antibody Fab fragment and double-stranded DNA diffract x-rays to 3.5-A resolution.

Mistargeting of peroxisomal L-alanine:glyoxylate aminotransferase to mitochondria in primary hyperoxaluria patients depends upon activation of a cryptic mitochondrial targeting sequence by a point mutation.

Evolution of behavior by density-dependent natural selection.

Identification of a cold shock transcriptional enhancer of the Escherichia coli gene encoding nucleoid protein H-NS.

Identification of the most common mutation within the porphobilinogen deaminase gene in Swedish patients with acute intermittent porphyria.

Simultaneous visualization of chromosome bands and hybridization signal using colloidal-gold labeling in electron microscopy.

Limited T-cell receptor beta-chain heterogeneity among interleukin 2 receptor-positive synovial T cells suggests a role for superantigen in rheumatoid arthritis.

Transmissible familial Creutzfeldt-Jakob disease associated with five, seven, and eight extra octapeptide coding repeats in the PRNP gene.

Neuropeptide Y secretion increases in the paraventricular nucleus in association with increased appetite for food.

Aniracetam reduces glutamate receptor desensitization and slows the decay of fast excitatory synaptic currents in the hippocampus.

Molecular cloning of a putative plant endomembrane protein resembling vertebrate protein disulfide-isomerase and a phosphatidylinositol-specific phospholipase C.

T cells expressing the V beta 1 T-cell receptor are required for IgA production in the chicken.

Two distinct alpha beta T-cell lineages can be distinguished by the differential usage of T-cell receptor V beta gene segments.

M-15: high-affinity chimeric peptide that blocks the neuronal actions of galanin in the hippocampus, locus coeruleus, and spinal cord.

Both activation and repression of a-mating-type-specific genes in yeast require transcription factor Mcm1.

Allelic variation in the effects of the nef gene on replication of human immunodeficiency virus type 1.

In vivo biodistribution of a radiolabeled blood substitute: 99mTc-labeled liposome-encapsulated hemoglobin in an anesthetized rabbit.

cDNA cloning, sequence analysis, and chromosomal localization of human carnitine palmitoyltransferase.

Year: 1991, Volume: 88, Issue: 22

Year: 1991, Volume: 88, Issue: 21

Year: 1991, Volume: 88, Issue: 20

Year: 1991, Volume: 88, Issue: 19

Year: 1991, Volume: 88, Issue: 18

Year: 1991, Volume: 88, Issue: 17

Year: 1991, Volume: 88, Issue: 16

Year: 1991, Volume: 88, Issue: 15

Year: 1991, Volume: 88, Issue: 14

Year: 1991, Volume: 88, Issue: 13

Year: 1991, Volume: 88, Issue: 12

Year: 1991, Volume: 88, Issue: 11

Year: 1991, Volume: 88, Issue: 10

Year: 1991, Volume: 88, Issue: 9

Year: 1991, Volume: 88, Issue: 8

Year: 1991, Volume: 88, Issue: 7

Year: 1991, Volume: 88, Issue: 6

Year: 1991, Volume: 88, Issue: 5

Year: 1991, Volume: 88, Issue: 4

Year: 1991, Volume: 88, Issue: 3

Year: 1991, Volume: 88, Issue: 2

Year: 1991, Volume: 88, Issue: 1

Year: 1990, Volume: 87

Year: 1989, Volume: 86

Year: 1988, Volume: 85

Year: 1987, Volume: 84

Year: 1986, Volume: 83

Year: 1985, Volume: 82

Year: 1984, Volume: 81

Year: 1983, Volume: 80

Year: 1982, Volume: 79

Year: 1981, Volume: 78

Year: 1980, Volume: 77

Year: 1979, Volume: 76

Year: 1978, Volume: 75

Year: 1977, Volume: 74

Year: 1976, Volume: 73

Year: 1975, Volume: 72

Year: 1974, Volume: 71

Year: 1973, Volume: 70

Year: 1972, Volume: 69

Year: 1971, Volume: 68

Year: 1970, Volume: 67

Year: 1970, Volume: 66

Year: 1970, Volume: 65

Year: 1969, Volume: 64

Year: 1969, Volume: 63

Year: 1969, Volume: 62

Year: 1968, Volume: 61

Year: 1968, Volume: 60

Year: 1968, Volume: 59

Year: 1967, Volume: 58

Year: 1967, Volume: 57

Year: 1966, Volume: 56

Year: 1966, Volume: 55

Year: 1965, Volume: 54

Year: 1965, Volume: 53

Year: 1964, Volume: 52

Year: 1964, Volume: 51

Year: 1963, Volume: 50

Year: 1963, Volume: 49

Year: 1962, Volume: 48

Year: 1961, Volume: 47

Year: 1960, Volume: 46

Year: 1959, Volume: 45

Year: 1958, Volume: 44

Year: 1957, Volume: 43

Year: 1956, Volume: 42

Year: 1955, Volume: 41

Year: 1954, Volume: 40

Year: 1953, Volume: 39

Year: 1952, Volume: 38

Year: 1951, Volume: 37

Year: 1950, Volume: 36

Year: 1949, Volume: 35

Year: 1948, Volume: 34

Year: 1947, Volume: 33

Year: 1946, Volume: 32

Year: 1945, Volume: 31

Year: 1944, Volume: 30

Year: 1943, Volume: 29

Year: 1942, Volume: 28

Year: 1941, Volume: 27

Year: 1940, Volume: 26

Year: 1939, Volume: 25

Year: 1938, Volume: 24

Year: 1937, Volume: 23

Year: 1936, Volume: 22

Year: 1935, Volume: 21

Year: 1934, Volume: 20

Year: 1933, Volume: 19

Year: 1932, Volume: 18

Year: 1931, Volume: 17

Year: 1930, Volume: 16

Year: 1929, Volume: 15

Year: 1928, Volume: 14

Year: 1927, Volume: 13

Year: 1926, Volume: 12

Year: 1925, Volume: 11

Year: 1924, Volume: 10

Year: 1923, Volume: 9

Year: 1922, Volume: 8

Year: 1921, Volume: 7

Year: 1920, Volume: 6

Year: 1919, Volume: 5

Year: 1918, Volume: 4

Year: 1917, Volume: 3

Year: 1916, Volume: 2

Year: 1915, Volume: 1

Phasing the conformational unit of spectrin.

Content Provider	PubMed Central
Author	Winograd, E. Hume, D. Branton, D.
Abstract	Many proteins contain a repetitive sequence motif, which implies that they contain a repetitive structural motif. Spectrin and the related proteins dystrophin and alpha-actinin consist largely of repeated motifs of 100-120 residues. But the repeating motif is degenerate and it has been difficult to define the boundaries of the repeating sequence unit or its corresponding structural unit. We have determined at which residues the structural units that correspond to spectrin's repeating 106-amino acid motifs begin and end. Drosophila alpha-spectrin cDNAs were expressed in bacteria to show that single segments (106 amino acids) and pairs of segments encoded by selected regions of spectrin cDNA can fold into stable conformations whose biophysical and biochemical properties are similar to those of native spectrin. Because such folding was critically dependent on the phasing of the expressed sequence with respect to the apparent boundaries of the repeating motifs, our data provide experimental evidence that relates the boundaries of the folded, conformational unit to the chemical sequence of repeating motifs.
Starting Page	10788
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	23
Volume Number	88
Language	English
Publisher Date	1991-12-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in