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| Content Provider | PubMed Central |
|---|---|
| Author | Bian, Dong-lin Wang, Xue-mei Huang, Kun Zhai, Qi-xi Yu, Gui-bo Wu, Cheng-hua |
| Copyright Year | 2016 |
| Abstract | The aim of the present study was to evaluate the expression level of microRNA-182 (miRNA-182) in human osteosarcoma (OS) MG-63 cells and OS tissues, and to elucidate the effect of miRNA-182 on the biological activity of tumors. In the present study, the expression of miRNA-182 in human OS MG-63 cells, OS tissues and normal osteoblast hFOB1.19 cells was determined using quantitative polymerase chain reaction. Subsequently, a miRNA-182 mimic and inhibitor were utilized to regulate the expression level of this miRNA in MG-63 cells. Cell viability and proliferation were examined using cell counting kit-8 assays, and cell apoptosis was detected by flow cytometry. Cell invasion and migration assays were performed using Transwell chambers to analyze the biological functions of miRNA-182 in vitro. The present study demonstrated that the expression level of miRNA-182 in MG-63 cells and OS tissues was significantly increased compared with the hFOB1.19 cell line (P<0.05). The present study successfully performed cell transfections of miRNA-182 inhibitor and miRNA-182 mimic into MG-63 cells and achieved the desired transfection efficiency. The present study confirmed that upregulation of miRNA-182 promotes cell apoptosis and inhibits cell viability, proliferation, invasion and migration. The present findings additionally demonstrated that miRNA-182 is a tumor suppressor gene in OS. Therefore, regulating the expression of miRNA-182 may affect the biological behavior of OS cells, which suggests a potential role for miRNA-182 in molecular therapy for malignant tumors. |
| Related Links | http://dx.doi.org/10.3892/ol.2016.4375 |
| Starting Page | 3040 |
| File Format | |
| ISSN | 17921074 |
| e-ISSN | 17921082 |
| Journal | Oncology Letters |
| Issue Number | 5 |
| Volume Number | 11 |
| Language | English |
| Publisher | D.A. Spandidos |
| Publisher Date | 2016-03-01 |
| Access Restriction | Open |
| Rights Holder | D.A. Spandidos |
| Subject Keyword | Cancer Research Oncology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Oncology |
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