NDLI: MHC class II tetramers identify peptide-specific human CD4+ T cells proliferating in response to influenza A antigen

Content Provider	PubMed Central
Author	Novak, Erik J. Liu, Andrew W. Nepom, Gerald T. Kwok, William W.
Copyright Year	1999
Abstract	Antigen-specific T helper cells present in peripheral blood at very low frequencies are capable of rapid clonal expansion during antigenic challenge. The exquisite specificity of this response provides for activation and expansion of a very select cohort of T cells, a feature we have used to directly identify and quantify human epitope-specific T helper cells from peripheral blood. Soluble tetramerized class II MHC molecules, loaded with an immunodominant peptide from hemagglutinin (HA) and labeled with fluorescent dyes, were constructed and used to directly identify antigen-specific T cells from influenza-immune individuals. After 7 days of proliferation in response to stimulation by HA peptide or whole influenza vaccine, cells staining positive with the HA tetramer had undergone between 6 and 9 divisions and were CD3+, CD4+, CD25+, and CD8–, characteristic of activated T helper cells responding to antigen. The HA epitope-specific component of the complex response to whole influenza vaccine represented a major subset of proliferating T helper cells. Soluble class II tetramers allow a direct approach for the analysis of immunodominant antigenic specificities. The identification of antigen-specific T helper cells in the peripheral blood provides a means for tracking the immune response against infectious agents and in autoimmune disease.
Related Links	http://dx.doi.org/10.1172/jci8476
Starting Page	63
File Format	PDF
ISSN	00219738
Journal	Journal of Clinical Investigation
Issue Number	12
Volume Number	104
Language	English
Publisher	American Society for Clinical Investigation
Publisher Date	1999-12-15
Access Restriction	Open
Rights Holder	American Society for Clinical Investigation
Subject Keyword	Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine

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