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| Content Provider | PubMed Central |
|---|---|
| Author | Valentin, Jossen Schirmer, Cedric Sindi, Dolman Mostafa Eibl, Regine Matthias, Kraume Ralf, Pörtner Eibl, Dieter |
| Copyright Year | 2016 |
| Abstract | The potential of human mesenchymal stem cells (hMSCs) for allogeneic cell therapies has created a large amount of interest. However, this presupposes the availability of efficient scale-up procedures. Promising results have been reported for stirred bioreactors that operate with microcarriers. Recent publications focusing on microcarrier-based stirred bioreactors have demonstrated the successful use of Computational Fluid Dynamics (CFD) and suspension criteria (N S1u , N S1) for rapidly scaling up hMSC expansions from mL- to pilot scale. Nevertheless, one obstacle may be the formation of large microcarrier-cell-aggregates, which may result in mass transfer limitations and inhomogeneous distributions of stem cells in the culture broth. The dependence of microcarrier-cell-aggregate formation on impeller speed and shear stress levels was investigated for human adipose derived stromal/stem cells (hASCs) at the spinner scale by recording the Sauter mean diameter (d 32) versus time. Cultivation at the suspension criteria provided d 32 values between 0.2 and 0.7 mm, the highest cell densities (1.25 × 106 cells mL−1 hASCs), and the highest expansion factors (117.0 ± 4.7 on day 7), while maintaining the expression of specific surface markers. Furthermore, suitability of the suspension criterion N S1u was investigated for scaling up microcarrier-based processes in wave-mixed bioreactors for the first time. |
| Related Links | http://dx.doi.org/10.1155/2016/4760414 |
| Starting Page | 4760414 |
| File Format | |
| ISSN | 1687966X |
| e-ISSN | 16879678 |
| Journal | Stem Cells International |
| Volume Number | 2016 |
| Language | English |
| Publisher | Hindawi Publishing Corporation |
| Publisher Date | 2016-01-01 |
| Access Restriction | Open |
| Rights Holder | Hindawi Publishing Corporation |
| Subject Keyword | Stem Cells International Healthcare Stem Cell genetics Medicine Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Molecular Biology |
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