NDLI: Meiotic Recombination in Neurospora crassa Proceeds by Two Pathways with Extensive Holliday Junction Migration

Content Provider	PubMed Central
Author	Yeadon, Patricia Jane Bowring, Frederick James Catcheside, David E. A.
Editor	Borkovich, Katherine A.
Copyright Year	2016
Abstract	Analysis of thousands of Δmsh-2 octads using our fluorescent recombination system indicates that, as in other filamentous fungi, symmetric heteroduplex is common in the his-3 region of Neurospora crassa. Symmetric heteroduplex arises from Holliday junction migration, and we suggest this mechanism explains the high frequency of His+ spores in heteroallelic crosses in which recombination is initiated cis to the his-3 allele further from the initiator, cog + . In contrast, when recombination is initiated cis to the his-3 allele closer to cog + , His+ spores are mainly a result of synthesis-dependent strand annealing, yielding asymmetric heteroduplex. Loss of Msh-2 function increases measures of allelic recombination in both his-3 and the fluorescent marker gene, indicating that mismatches in asymmetric heteroduplex, as in Saccharomyces cerevisiae, tend to be repaired in the direction of restoration. Furthermore, the presence of substantial numbers of conversion octads in crosses lacking Msh-2 function suggests that the disjunction pathway described in S. cerevisiae is also active in Neurospora, adding to evidence for a universal model for meiotic recombination.
Related Links	http://dx.doi.org/10.1371/journal.pone.0147815
Starting Page	147815
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	1
Volume Number	11
Language	English
Publisher	Public Library of Science
Publisher Date	2016-01-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Top3α Is Required during the Convergent Migration Step of Double Holliday Junction Dissolution

Recombination-independent recognition of DNA homology for meiotic silencing in Neurospora crassa

Systems Biology of the Clock in Neurospora crassa

Characterization of Apoptosis-Related Oxidoreductases from Neurospora crassa

Systems Biology of the qa Gene Cluster in Neurospora crassa

Relationship between Phylogenetic Distribution and Genomic Features in Neurospora crassa

Gene Expression Differences among Three Neurospora Species Reveal Genes Required for Sexual Reproduction in Neurospora crassa

Identification of the CRE-1 Cellulolytic Regulon in Neurospora crassa

Direct Transcriptional Control of a p38 MAPK Pathway by the Circadian Clock in Neurospora crassa

Meiotic Recombination in Neurospora crassa Proceeds by Two Pathways with Extensive Holliday Junction Migration

Similar Documents

Top3α Is Required during the Convergent Migration Step of Double Holliday Junction Dissolution

Recombination-independent recognition of DNA homology for meiotic silencing in Neurospora crassa

Systems Biology of the Clock in Neurospora crassa

Characterization of Apoptosis-Related Oxidoreductases from Neurospora crassa

Systems Biology of the qa Gene Cluster in Neurospora crassa

Relationship between Phylogenetic Distribution and Genomic Features in Neurospora crassa

Gene Expression Differences among Three Neurospora Species Reveal Genes Required for Sexual Reproduction in Neurospora crassa

Identification of the CRE-1 Cellulolytic Regulon in Neurospora crassa

Direct Transcriptional Control of a p38 MAPK Pathway by the Circadian Clock in Neurospora crassa

Meiotic Recombination in Neurospora crassa Proceeds by Two Pathways with Extensive Holliday Junction Migration