NDLI: Persistence of F-Specific RNA Coliphages in Surface Waters from a Produce Production Region along the Central Coast of California

Content Provider	PubMed Central
Author	Ravva, Subbarao V. Sarreal, Chester Z.
Editor	Balcazar, Jose Luis
Abstract	F+ RNA coliphages (FRNA) are used to source-track fecal contamination and as surrogates for enteric pathogen persistence in the environment. However, the environmental persistence of FRNA is not clearly understood and necessitates the evaluation of the survival of prototype and environmental isolates of FRNA representing all four genogroups in surface waters from the central coast of California. Water temperature played a significant role in persistence–all prototype and environmental strains survived significantly longer at 10°C compared to 25°C. Similarly, the availability of host bacterium was found to be critical in FRNA survival. In the absence of E. coli Famp, all prototypes of FRNA disappeared rapidly with a D-value (days for one log reduction) of <1.2 d from water samples incubated at 25°C; the longest surviving prototype was SP. However, in the presence of the host, the order of persistence at 25°C was QB>MS2>SP>GA and at 10°C it was QB = MS2>GA>SP. Significant differences in survival were observed between prototypes and environmental isolates of FRNA. While most environmental isolates disappeared rapidly at 25°C and in the absence of the host, members of genogroups GIII and GI persisted longer with the host compared to members of GII and GIV. Consequentially, FRNA based source tracking methods can be used to detect phages from recent fecal contamination along with those that persist longer in the environment as a result of cooler temperatures and increased host presence.
Related Links	http://dx.doi.org/10.1371/journal.pone.0146623
Starting Page	146623
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	1
Volume Number	11
Language	English
Publisher	Public Library of Science
Publisher Date	2016-01-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Incidence and Tracking of Escherichia coli O157:H7 in a Major Produce Production Region in California

Sea Surface Temperature Influence on Terrestrial Gross Primary Production along the Southern California Current

Phylogeography of Supralittoral Rocky Intertidal Ligia Isopods in the Pacific Region from Central California to Central Mexico

Identification of Surface Water Quality along the Coast of Sanya, South China Sea

Stranding Events of Kogia Whales along the Brazilian Coast

Twelve Years of Change in Coastal Upwelling along the Central-Northern Coast of Chile: Spatially Heterogeneous Responses to Climatic Variability

Phylogeography of Ostreopsis along West Pacific Coast, with Special Reference to a Novel Clade from Japan

Climatic Control of Upwelling Variability along the Western North-American Coast

Vulnerability of Mediterranean Ecosystems to Long-Term Changes along the Coast of Israel

Persistence of F-Specific RNA Coliphages in Surface Waters from a Produce Production Region along the Central Coast of California

Similar Documents

Incidence and Tracking of Escherichia coli O157:H7 in a Major Produce Production Region in California

Sea Surface Temperature Influence on Terrestrial Gross Primary Production along the Southern California Current

Phylogeography of Supralittoral Rocky Intertidal Ligia Isopods in the Pacific Region from Central California to Central Mexico

Identification of Surface Water Quality along the Coast of Sanya, South China Sea

Stranding Events of Kogia Whales along the Brazilian Coast

Twelve Years of Change in Coastal Upwelling along the Central-Northern Coast of Chile: Spatially Heterogeneous Responses to Climatic Variability

Phylogeography of Ostreopsis along West Pacific Coast, with Special Reference to a Novel Clade from Japan

Climatic Control of Upwelling Variability along the Western North-American Coast

Vulnerability of Mediterranean Ecosystems to Long-Term Changes along the Coast of Israel

Persistence of F-Specific RNA Coliphages in Surface Waters from a Produce Production Region along the Central Coast of California