NDLI: Chemosensitization of Cancer Cells via Gold Nanoparticle-Induced Cell Cycle Regulation

Content Provider	PubMed Central
Author	Mackey, Megan A. El Sayed, Mostafa A.
Abstract	We have previously shown that plasmonic nanoparticles conjugated with nuclear-targeting and cytoplasm-targeting peptides (NLS and RGD, respectively) are capable of altering the cell cycle of human oral squamous carcinoma cells (HSC-3). In the present work, we show that this regulation of the cell cycle can be exploited to enhance the efficacy of a common chemotherapeutic agent, 5-Fluorouracil, by pre-treating cells with gold nanoparticles. Utilizing flow cytometry cell cycle analysis, we were able to quantify the 5-Fluorouracil efficacy as an accumulation of cells in the S phase with a depletion of cells in the G2/M phase. Two gold nanoparticle sizes were tested in this work; 30 nm with a surface plasmon resonance at 530 nm and 15 nm with a surface plasmon resonance at 520 nm. The 30 nm nuclear-targeted gold nanoparticles (NLS-AuNPs) showed the greatest 5-Fluorouracil efficacy enhancement when 5-Fluorouracil treatment (500 μM, 48 h) is preceded by a 24 h treatment with nanoparticles. In conclusion, we show that nuclear-targeted 30 nm gold nanoparticles enhance 5-Fluorouracil drug efficacy in HSC-3 cells via regulation of the cell cycle, a chemosensitization technique that could potentially be expanded to different cell lines and different chemotherapies.
Related Links	http://dx.doi.org/10.1111/php.12226
Ending Page	312
Page Count	7
Starting Page	306
File Format	PDF
ISSN	17511097
e-ISSN	17511097
Journal	Photochemistry and photobiology
Issue Number	2
Volume Number	90
Language	English
Publisher Date	2014-01-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Physical and Theoretical Chemistry Radiation

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