NDLI: Weak Interactions between Salmonella enterica FlhB and Other Flagellar Export Apparatus Proteins Govern Type III Secretion Dynamics

Content Provider	PubMed Central
Author	Mcmurry, Jonathan L. Minamino, Tohru Furukawa, Yukio Francis, Joshua W. Hill, Stephanie A. Helms, Katy A. Namba, Keiichi
Editor	Hensel, Michael
Copyright Year	2015
Abstract	The bacterial flagellum contains its own type III secretion apparatus that coordinates protein export with assembly at the distal end. While many interactions among export apparatus proteins have been reported, few have been examined with respect to the differential affinities and dynamic relationships that must govern the mechanism of export. FlhB, an integral membrane protein, plays critical roles in both export and the substrate specificity switching that occurs upon hook completion. Reported herein is the quantitative characterization of interactions between the cytoplasmic domain of FlhB (FlhBC) and other export apparatus proteins including FliK, FlhAC and FliI. FliK and FlhAC bound with micromolar affinity. KD for FliI binding in the absence of ATP was 84 nM. ATP-induced oligomerization of FliI induced kinetic changes, stimulating fast-on, fast-off binding and lowering affinity. Full length FlhB purified under solubilizing, nondenaturing conditions formed a stable dimer via its transmembrane domain and stably bound FliH. Together, the present results support the previously hypothesized central role of FlhB and elucidate the dynamics of protein-protein interactions in type III secretion.
Related Links	http://dx.doi.org/10.1371/journal.pone.0134884
Starting Page	134884
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	8
Volume Number	10
Language	English
Publisher	Public Library of Science
Publisher Date	2015-08-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Weak interactions between salmonella enterica flhb and other flagellar export apparatus proteins govern type iii secretion dynamics.

Weak Interactions between Salmonella enterica FlhB and Other Flagellar Export Apparatus Proteins Govern Type III Secretion Dynamics

Research article weak interactions between salmonella enterica flhb and other flagellar export apparatus proteins govern type iii secretion dynamics (1371).

Cross-Complementation Study of the Flagellar Type III Export Apparatus Membrane Protein FlhB

Function of FlhB, a Membrane Protein Implicated in the Bacterial Flagellar Type III Secretion System

Cross-Complementation Study of the Flagellar Type III Export Apparatus Membrane Protein FlhB

Analysis of an engineered Salmonella flagellar fusion protein (2004)

Analysis of the Expression, Secretion and Translocation of the Salmonella enterica Type III Secretion System Effector SteA

Interactions of flij with the salmonella type iii flagellar export apparatus (2003).

Weak Interactions between Salmonella enterica FlhB and Other Flagellar Export Apparatus Proteins Govern Type III Secretion Dynamics

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Weak interactions between salmonella enterica flhb and other flagellar export apparatus proteins govern type iii secretion dynamics.

Weak Interactions between Salmonella enterica FlhB and Other Flagellar Export Apparatus Proteins Govern Type III Secretion Dynamics

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