NDLI: Generation of Viable Plant-Vertebrate Chimeras

Content Provider	PubMed Central
Author	Alvarez, Marjorie Reynaert, Nicole Chávez, Myra N. Aedo, Geraldine Araya, Francisco Hopfner, Ursula Fernández, Juan Allende, Miguel L. Egaña, José T.
Editor	Solé, Ricard V.
Copyright Year	2015
Abstract	The extreme dependence on external oxygen supply observed in animals causes major clinical problems and several diseases are related to low oxygen tension in tissues. The vast majority of the animals do not produce oxygen but a few exceptions have shown that photosynthetic capacity is physiologically compatible with animal life. Such symbiotic photosynthetic relationships are restricted to a few aquatic invertebrates. In this work we aimed to explore if we could create a chimerical organism by incorporating photosynthetic eukaryotic cells into a vertebrate animal model. Here, the microalgae Chlamydomonas reinhardtii was injected into zebrafish eggs and the interaction and viability of both organisms were studied. Results show that microalgae were distributed into different tissues, forming a fish-alga chimera organism for a prolonged period of time. In addition, microscopic observation of injected algae, in vivo expression of their mRNA and re-growth of the algae ex vivo suggests that they survived to the developmental process, living for several days after injection. Moreover microalgae did not trigger a significant inflammatory response in the fish. This work provides additional evidence to support the possibility that photosynthetic vertebrates can be engineered.
Related Links	http://dx.doi.org/10.1371/journal.pone.0130295
Starting Page	130295
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	6
Volume Number	10
Language	English
Publisher	Public Library of Science
Publisher Date	2015-06-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Systematic Characteristic Exploration of the Chimeras Generated in Multiple Displacement Amplification through Next Generation Sequencing Data Reanalysis

Organizational Heterogeneity of Vertebrate Genomes

Efficient Generation of Germ Line Transmitting Chimeras from C57BL/6N ES Cells by Aggregation with Outbred Host Embryos

Availability of Empty Zona Pellucida for Generating Embryonic Chimeras

A Membrane-Bound Vertebrate Globin

Evolution of Mitochondrial Power in Vertebrate Metazoans

Effect of Glycyrrhizin on Pseudomonal Skin Infections in Human-Mouse Chimeras

Control of Vertebrate Skeletal Mineralization by Polyphosphates

Evolution of the Vertebrate Resistin Gene Family

Generation of Viable Plant-Vertebrate Chimeras

Similar Documents

Systematic Characteristic Exploration of the Chimeras Generated in Multiple Displacement Amplification through Next Generation Sequencing Data Reanalysis

Organizational Heterogeneity of Vertebrate Genomes

Efficient Generation of Germ Line Transmitting Chimeras from C57BL/6N ES Cells by Aggregation with Outbred Host Embryos

Availability of Empty Zona Pellucida for Generating Embryonic Chimeras

A Membrane-Bound Vertebrate Globin

Evolution of Mitochondrial Power in Vertebrate Metazoans

Effect of Glycyrrhizin on Pseudomonal Skin Infections in Human-Mouse Chimeras

Control of Vertebrate Skeletal Mineralization by Polyphosphates

Evolution of the Vertebrate Resistin Gene Family

Generation of Viable Plant-Vertebrate Chimeras