NDLI: Phosphotransferase System-Dependent Extracellular Growth of Listeria monocytogenes Is Regulated by Alternative Sigma Factors σL and σH

Content Provider	PubMed Central
Author	Wang, Siyun Orsi, Renato H. Tang, Silin Zhang, Wei Wiedmann, Martin Boor, Kathryn J.
Copyright Year	2014
Abstract	Alternative sigma (σ) factors and phosphotransferase systems (PTSs) play pivotal roles in the environmental adaptation and virulence of Listeria monocytogenes. The growth of the L. monocytogenes parent strain 10403S and 15 isogenic alternative σ factor mutants was assessed in defined minimal medium (DM) with PTS-dependent or non-PTS-dependent carbon sources at 25°C or 37°C. Overall, our results suggested that the regulatory effect of alternative σ factors on the growth of L. monocytogenes is dependent on the temperature and the carbon source. One-way analysis of variance (one-way ANOVA) showed that the factor “strain” had a significant effect on the maximum growth rate (μmax), lag phase duration (λ), and maximum optical density (ODmax) in PTS-dependent carbon sources (P < 0.05) but not in a non-PTS-dependent carbon source. Also, the ODmax was not affected by strain for any of the three PTS-dependent carbon sources at 25°C but was affected by strain at 37°C. Monitoring by quantitative real-time PCR (qRT-PCR) showed that transcript levels for lmo0027, a glucose-glucoside PTS permease (PTSGlc-1)-encoding gene, were higher in the absence of σL, and lower in the absence of σH, than in the parent strain. Our data thus indicate that σL negatively regulates lmo0027 and that the increased μmax observed for the ΔsigL strain in DM with glucose may be associated with increased expression of PTSGlc-1 encoded by lmo0027. Our findings suggest that σH and σL mediate the PTS-dependent growth of L. monocytogenes through complex transcriptional regulations and fine-tuning of the expression of specific pts genes, including lmo0027. Our findings also reveal a more important and complex role of alternative σ factors in the regulation of growth in different sugar sources than previously assumed.
Related Links	http://dx.doi.org/10.1128/AEM.02530-14
Ending Page	7682
Page Count	10
Starting Page	7673
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	24
Volume Number	80
Language	English
Publisher	American Society for Microbiology
Publisher Date	2014-12-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

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