NDLI: Hydroxycinnamic Acids Used as External Acceptors of Electrons: an Energetic Advantage for Strictly Heterofermentative Lactic Acid Bacteria

Content Provider	PubMed Central
Author	Pasquale, Filannino Marco, Gobbetti Angelis, Maria De Cagno, Raffaella Di
Editor	Elkins, C. A.
Copyright Year	2014
Abstract	The metabolism of hydroxycinnamic acids by strictly heterofermentative lactic acid bacteria (19 strains) was investigated as a potential alternative energy route. Lactobacillus curvatus PE5 was the most tolerant to hydroxycinnamic acids, followed by strains of Weissella spp., Lactobacillus brevis, Lactobacillus fermentum, and Leuconostoc mesenteroides, for which the MIC values were the same. The highest sensitivity was found for Lactobacillus rossiae strains. During growth in MRS broth, lactic acid bacteria reduced caffeic, p-coumaric, and ferulic acids into dihydrocaffeic, phloretic, and dihydroferulic acids, respectively, or decarboxylated hydroxycinnamic acids into the corresponding vinyl derivatives and then reduced the latter compounds to ethyl compounds. Reductase activities mainly emerged, and the activities of selected strains were further investigated in chemically defined basal medium (CDM) under anaerobic conditions. The end products of carbon metabolism were quantified, as were the levels of intracellular ATP and the NAD+/NADH ratio. Electron and carbon balances and theoretical ATP/glucose yields were also estimated. When CDM was supplemented with hydroxycinnamic acids, the synthesis of ethanol decreased and the concentration of acetic acid increased. The levels of these metabolites reflected on the alcohol dehydrogenase and acetate kinase activities. Overall, some biochemical traits distinguished the common metabolism of strictly heterofermentative strains: main reductase activity toward hydroxycinnamic acids, a shift from alcohol dehydrogenase to acetate kinase activities, an increase in the NAD+/NADH ratio, and the accumulation of supplementary intracellular ATP. Taken together, the above-described metabolic responses suggest that strictly heterofermentative lactic acid bacteria mainly use hydroxycinnamic acids as external acceptors of electrons.
Related Links	http://dx.doi.org/10.1128/AEM.02413-14
Ending Page	7582
Page Count	9
Starting Page	7574
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	24
Volume Number	80
Language	English
Publisher	American Society for Microbiology
Publisher Date	2014-12-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Heterofermentative lactic acid bacteria dominate in Korean commercial kimchi

A Differential Medium for the Enumeration of Homofermentative and Heterofermentative Lactic Acid Bacteria †

Characteristics of Some Lactic Acid Bacteria Used as Starter Cultures in Dry Sausage Production

Animal Rennets as Sources of Dairy Lactic Acid Bacteria

Ability of Thermophilic Lactic Acid Bacteria To Produce Aroma Compounds from Amino Acids

Lactic Acid Bacteria as a Potential Source of Enzymes for Use in Vinification

Comparative Genomics of Enzymes in Flavor-Forming Pathways from Amino Acids in Lactic Acid Bacteria▿ †

Use of the alr Gene as a Food-Grade Selection Marker in Lactic Acid Bacteria

9th International Symposium on Lactic Acid Bacteria▿

Hydroxycinnamic Acids Used as External Acceptors of Electrons: an Energetic Advantage for Strictly Heterofermentative Lactic Acid Bacteria

Similar Documents

Heterofermentative lactic acid bacteria dominate in Korean commercial kimchi

A Differential Medium for the Enumeration of Homofermentative and Heterofermentative Lactic Acid Bacteria †

Characteristics of Some Lactic Acid Bacteria Used as Starter Cultures in Dry Sausage Production

Animal Rennets as Sources of Dairy Lactic Acid Bacteria

Ability of Thermophilic Lactic Acid Bacteria To Produce Aroma Compounds from Amino Acids

Lactic Acid Bacteria as a Potential Source of Enzymes for Use in Vinification

Comparative Genomics of Enzymes in Flavor-Forming Pathways from Amino Acids in Lactic Acid Bacteria▿ †

Use of the alr Gene as a Food-Grade Selection Marker in Lactic Acid Bacteria

9th International Symposium on Lactic Acid Bacteria▿

Hydroxycinnamic Acids Used as External Acceptors of Electrons: an Energetic Advantage for Strictly Heterofermentative Lactic Acid Bacteria