NDLI: Pyrophosphate-Mediated Iron Acquisition from Transferrin in Neisseriameningitidis Does Not Require TonB Activity

Content Provider	PubMed Central
Author	Francis, Biville Christophe, Brézillon Giorgini, Dario Taha, Muhamed-kheir
Editor	Cascales, Eric
Copyright Year	2014
Abstract	The ability to acquire iron from various sources has been demonstrated to be a major determinantin the pathogenesis of Neisseria meningitidis. Outside the cells, iron is bound totransferrin in serum, or to lactoferrin in mucosal secretions. Meningococci can extract iron fromiron-loaded human transferrin by the TbpA/TbpB outer membrane complex. Moreover, N.meningitidis expresses the LbpA/LbpB outer membrane complex, which can extract iron fromiron-loaded human lactoferrin. Iron transport through the outer membrane requires energy provided bythe ExbB-ExbD-TonB complex. After transportation through the outer membrane, iron is bound byperiplasmic protein FbpA and is addressed to the FbpBC inner membrane transporter. Iron-complexingcompounds like citrate and pyrophosphate have been shown to support meningococcal growth exvivo. The use of iron pyrophosphate as an iron source by N. meningitidiswas previously described, but has not been investigated. Pyrophosphate was shown to participate iniron transfer from transferrin to ferritin. In this report, we investigated the use of ferricpyrophosphate as an iron source by N. meningitidis both ex vivoand in a mouse model. We showed that pyrophosphate was able to sustain N.meningitidis growth when desferal was used as an iron chelator. Addition of a pyrophosphateanalogue to bacterial suspension at millimolar concentrations supported N.meningitidis survival in the mouse model. Finally, we show that pyrophosphate enabledTonB-independent ex vivo use of iron-loaded human or bovine transferrin as an ironsource by N. meningitidis. Our data suggest that, in addition to acquiring ironthrough sophisticated systems, N. meningitidis is able to use simple strategies toacquire iron from a wide range of sources so as to sustain bacterial survival.
Related Links	http://dx.doi.org/10.1371/journal.pone.0107612
Starting Page	107612
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	10
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-10-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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