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Content Provider | PubMed Central |
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Author | Rana, Vandana Eckard, Anahita D. Ahring, Birgitte K. |
Abstract | The aim of the present study was to compare bioethanol production from wet exploded corn stover (WECS) and loblolly pine (WELP) hydrolyzed with in-house and commercial enzymes and fermented separately (SHF) and simultaneously (SSF). In-house enzymes produced from Trichoderma reesei, RUT-C30 and a novel fungal strain, Aspergillus saccharolyticus were loaded as 5 and 15 FPU/g glucan and supplemented with 10 and 30 CBU/g glucan, respectively. For hydrolysis and fermentation, slurries of WECS and WELP at 5 and 10% (w/w) solids loading (SL) were utilized. Saccharomyces cerevisae was used for ethanol fermentation at 33°C. Maximally, 15.6 g/L and 13.4 g/L (corresponding to theoretical ethanol yield of 76% and 67%, respectively) were achieved in SSF process from WECS and WELP, respectively at 5% SL and 15 FPU/g glucan loading of in-house enzymes. Ethanol concentrations in all cases were higher for SSF compared to SHF under same conditions. A cross comparison of SSF with commercial enzymes (Celluclast 1.5 L + Novozym 188) showed highest ethanol concentration of 17.3 g/L and 15.4 g/L (corresponding to theoretical ethanol yield of 84% and 77%, respectively) from WECS and WELP, respectively at 5% SL and 15 FPU/g glucan. These findings demonstrated that in-house enzymes were comparable to commercial enzymes as these fungi produced other lignocellulolytic enzymes beyond cellulase and hence enhanced the overall enzyme activity. |
Related Links | http://dx.doi.org/10.1186/2193-1801-3-516 |
Starting Page | 516 |
File Format | |
ISSN | 21931801 |
e-ISSN | 21931801 |
Journal | SpringerPlus |
Volume Number | 3 |
Language | English |
Publisher | Springer International Publishing |
Publisher Date | 2014-01-01 |
Access Restriction | Open |
Rights Holder | Springer International Publishing |
Subject Keyword | Research in Higher Education |
Content Type | Text |
Resource Type | Article |
Subject | Multidisciplinary |
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