NDLI: Whole-Mount X-Gal Staining of Mouse Tissues

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Content Provider	PubMed Central
Author	Gierut, Jessica J. Jacks, Tyler E. Haigis, Kevin M.
Copyright Year	2014
Abstract	Although the development of improved mouse models, including conditional deletions, marks an exciting time in mouse genetics, it is important to characterize and validate these models. Cre reporter strains allow researchers to assess the recombinase expression profile and function in individual Cre mouse lines. These strains are engineered to express a reporter gene (usually LacZ) following the removal of a floxed STOP cassette, thus marking cell lineages that can be targeted with a given Cre line. This protocol provides a detailed method for the histochemical detection of β-galactosidase activity in Cre mouse strains.
Related Links	http://dx.doi.org/10.1101/pdb.prot073452
Ending Page	419
Page Count	3
Starting Page	417
File Format	PDF
ISSN	15596095
e-ISSN	15596095
Journal	Cold Spring Harbor protocols
Issue Number	4
Volume Number	2014
Language	English
Publisher Date	2014-04-01
Access Restriction	Open
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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Whole-Mount X-Gal Staining of Mouse Tissues