NDLI: Whole-mount Staining of Mouse Diaphragm Neuromuscular Junctions.

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Whole-mount Staining of Mouse Diaphragm Neuromuscular Junctions.

Content Provider	Europe PMC
Author	Sha, Rula Wang, Zhenzhen You, Xue Liu, Yuguo Xie, Zhiqin Feng, Ying
Copyright Year	2021
Abstract	The neuromuscular junction (NMJ) is a specialized synapse that connects the terminal end of a motor neuron and a skeletal muscle fiber. Defects in NMJ cause abnormalities of neuromuscular transmission, leading to NMJ disorders. The mammalian diaphragm muscle is essential for respiration and has been widely used to study NMJ formation. Here, we provide a simple and straightforward protocol for preparing diaphragms from embryonic, neonatal, and adult mice and for subsequent NMJ staining.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC8595417&blobtype=pdf
Volume Number	11
PubMed Central reference number	PMC8595417
Issue Number	21
PubMed reference number	34859130
Journal	Bio-protocol [Bio Protoc]
e-ISSN	23318325
DOI	10.21769/bioprotoc.4215
Language	English
Publisher	Bio-Protocol
Publisher Date	2021-11-05
Publisher Place	1075 Lorne Way, Sunnyvale, CA 94087, USA
Access Restriction	Open
Rights License	Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.
Subject Keyword	Whole-mount staining Diaphragm NMJ Immunofluorescence
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Neuroscience Plant Science Biochemistry, Genetics and Molecular Biology

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