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| Content Provider | PubMed Central |
|---|---|
| Author | Neofytou, Evgenios A. Chang, Edwin Bhagat, Patlola Joubert, Lydia-marie Jayakumar, Rajadas Gambhir, Sanjiv S. Cheng, Zhen Robbins, Robert C. Beygui, Ramin E. |
| Copyright Year | 2011 |
| Abstract | Ischemic heart disease is the leading cause of death worldwide. Recent studies suggest that adipose tissue-derived stem cells (ASCs) can be used as a potential source for cardiovascular tissue engineering due to their ability to differentiate along the cardiovascular lineage and to adopt a proangiogenic phenotype. To understand better ASCs’ biology, we used a novel 3D culture device. ASCs’ and b.END-3 endothelial cell proliferation, migration, and vessel morphogenesis were significantly enhanced compared to 2D culturing techniques. ASCs were isolated from inguinal fat pads of 6-week-old GFP+/BLI+ mice. Early passage ASCs cells (P3-P4), PKH26-labeled murine b.END-3 cells or a co-culture of ASCs and b.END-3 cells were seeded at a density of 1 × 105 on three different surface configurations: (a) a 2D surface of tissue culture plastic, (b) Matrigel, and (c) a highly porous 3D scaffold fabricated from inert polystyrene. VEGF expression, cell proliferation, and tubulization, were assessed using optical microscopy, fluorescence microscopy, 3D confocal microscopy, and SEM imaging (n = 6). Increased VEGF levels were seen in conditioned media harvested from co-cultures of ASCs and b.END-3 on either Matrigel or a 3D matrix. Fluorescence, confocal, SEM, bioluminescence revealed improved cell, proliferation, and tubule formation for cells seeded on the 3D polystyrene matrix. Collectively, these data demonstrate that co-culturing ASCs with endothelial cells in a 3D matrix environment enable us to generate prevascularized tissue-engineered constructs. This can potentially help us to surpass the tissue thickness limitations faced by the tissue engineering community today. |
| Related Links | http://dx.doi.org/10.1002/jbm.a.33113 |
| Ending Page | 393 |
| Page Count | 11 |
| Starting Page | 383 |
| File Format | |
| ISSN | 15493296 |
| e-ISSN | 15524965 |
| Journal | Journal of biomedical materials research. Part A |
| Issue Number | 3 |
| Volume Number | 98 |
| Language | English |
| Publisher Date | 2011-09-01 |
| Access Restriction | Open |
| Subject Keyword | Medicine(all) Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ceramics and Composites Metals and Alloys Biomaterials Biomedical Engineering |
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