NDLI: In vivo multimodal microscopy for detecting bone-marrow-derived cell contribution to skin regeneration

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In vivo multimodal microscopy for detecting bone-marrow-derived cell contribution to skin regeneration

Content Provider	PubMed Central
Author	Graf, Benedikt W. Bower, Andrew J. Chaney, Eric J. Marjanovic, Marina Adie, Steven G. De Lisio, Michael Valero, Maria C. Boppart, Marni D. Boppart, Stephen A.
Copyright Year	2014
Abstract	Bone-marrow (BM)-derived cells have been shown to be capable of aiding skin regeneration in vivo by differentiating into keratinocytes. However, the conditions under which this occurs are not fully understood. Characterizing innate mechanisms of skin regeneration by stem cells in vivo is important for the area of stem cell biology. In this study, we investigate the use of novel in vivo imaging technology for characterizing the contribution of BM-derived cells to regeneration of the epidermis in mouse skin in vivo. In vivo imaging provides the ability to non-invasively observe the spatial positions and morphology of the BM-derived cells. Using a GFP BM-transplanted mouse model and in vivo multimodal microscopy, BM-derived cells can be observed in the skin. Our in vivo imaging method was used to search for the presence and identify the 3D spatial distribution of BM-derived cells in the epidermis of the skin under normal conditions, following wound healing, and after syngeneic skin grafting. We did not observe any evidence of BM-derived keratinocytes under these conditions, but we did observe BM-derived dendritic cells in the skin grafts. In vivo multimodal imaging has great potential for characterizing the conditions under which BM-derived cells contribute to skin regeneration. Multimodal in vivo images of a skin graft on a GFP bone-marrow-transplanted recipient mouse seven days after grafting. Modalities include (A) optical coherence tomography (OCT), (B) microvascular phase-variance OCT, (C) second harmonic generation (SHG), and (D) two-photon-excited fluorescence (TPEF) microscopy.
Related Links	http://dx.doi.org/10.1002/jbio.201200240
Ending Page	102
Page Count	7
Starting Page	96
File Format	PDF
ISSN	1864063X
e-ISSN	18640648
Journal	Journal of biophotonics
Issue Number	0
Volume Number	7
Language	English
Publisher Date	2014-01-01
Access Restriction	Open
Subject Keyword	Engineering(all) Biochemistry, Genetics and Molecular Biology(all) Physics and Astronomy(all) Materials Science(all) Chemistry(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Physics and Astronomy Biochemistry, Genetics and Molecular Biology Engineering Materials Science

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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