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Gut Colonization by Candida albicans Inhibits the Inductionof Humoral Immune Tolerance to Dietary Antigen in BALB/c Mice
| Content Provider | PubMed Central |
|---|---|
| Author | Sugita, Ryusuke Hata, Erina Miki, Atsuko Andoh, Ryoko Umeda, Chisato Takemura, Naoki Kei, Sonoyama |
| Copyright Year | 2012 |
| Abstract | We previously observed that gut colonization by Candida albicanspromoted serum antibody response to orally administered ovalbumin in mice. We thereforepostulated that C. albicans affects oral tolerance induction. The presentstudy tested this idea. BALB/c mice were intragastrically administered with eitherC. albicans (1 × 107) or vehicle, and the colonization wasconfirmed by weekly fecal cultures. Mice were further divided into two subgroups andintragastrically administered with either ovalbumin (20 mg) or vehicle for fiveconsecutive days. Thereafter, all mice were intraperitoneally immunized with ovalbumin inalum. In mice without C. albicans inoculation, ovalbumin feeding prior toimmunization significantly suppressed the increase in ovalbumin-specific IgE, IgG1 andIgG2a in sera, suggesting oral tolerance induction. In C.albicans-inoculated mice, however, the antibody levels were the same betweenovalbumin- and vehicle-fed mice. In contrast, ovalbumin feeding significantly suppressedcellular immune responses, as evidenced by reduced proliferation of splenocytesrestimulated by ovalbumin ex vivo, in both C.albicans-inoculated and uninoculated mice. Ex vivosupplementation with neither heat-killed C. albicans nor the culturesupernatant of C. albicans enhanced the production of ovalbumin-specificIgG1 in splenocytes restimulated by the antigen. These results suggest that gutcolonization by C. albicans inhibits the induction of humoral immunetolerance to dietary antigen in mice, whereas C. albicans may notdirectly promote antibody production. We therefore propose that C.albicans gut colonization could be a risk factor for triggering food allergy insusceptible individuals. |
| Related Links | http://dx.doi.org/10.12938/bmfh.31.77 |
| Ending Page | 84 |
| Page Count | 8 |
| Starting Page | 77 |
| File Format | |
| ISSN | 21863342 |
| e-ISSN | 21863342 |
| Journal | Bioscience of Microbiota, Food and Health |
| Issue Number | 4 |
| Volume Number | 31 |
| Language | English |
| Publisher | Bioscience of Microbiota, Food and Health |
| Publisher Date | 2012-01-01 |
| Access Restriction | Open |
| Rights Holder | Bioscience of Microbiota, Food and Health |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Gastroenterology Immunology Microbiology Food Science Applied Microbiology and Biotechnology |
