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| Content Provider | PubMed Central |
|---|---|
| Author | Wiederkehr, Rodrigo S. Mendes, Sergio B. |
| Copyright Year | 2013 |
| Abstract | We report here the fabrication, characterization, and application of asingle-mode integrated optical waveguide (IOW) spectrometer capable of acquiringoptical absorbance spectra of surface-immobilized molecules in the visible andultraviolet spectral region down to 315 nm. The UV-extension of the single-modeIOW technique to shorter wavelengths was made possible by our development of alow-loss single-mode dielectric waveguide in the UV region based on an aluminafilm grown by atomic layer deposition (ALD) over a high quality fused silicasubstrate, and by our design/fabrication of a broadband waveguide coupler formedby an integrated diffraction grating combined with a highly anamorphic opticalbeam of large numerical aperture. As an application of the developed technology,we report here the surface adsorption process of bacteriochlorophylla on different interfaces using its Soret absorption bandcentred at 370 nm. The effects of different chemical compositions at thesolid/liquid interface on the adsorption and spectral properties ofbacteriochlorophyll a were determined from the polarized UV-VisIOW spectra acquired with the developed instrumentation. The spectral extensionof the single-mode IOW technique into the ultraviolet region is an importantadvance as it enables extremely sensitive studies in key characteristics ofsurface molecular processes (e.g., protein unfolding and solvation of aromaticamino-acid groups under surface binding) whose spectral features are mainlylocated at wavelengths below the visible spectrum. |
| Related Links | http://dx.doi.org/10.1039/c3an02201c |
| Ending Page | 1402 |
| Page Count | 7 |
| Starting Page | 1396 |
| File Format | |
| ISSN | 00032654 |
| e-ISSN | 13645528 |
| Journal | The Analyst |
| Issue Number | 6 |
| Volume Number | 139 |
| Language | English |
| Publisher Date | 2014-01-01 |
| Access Restriction | Open |
| Subject Keyword | Analytical Chemistry Spectroscopy Electrochemistry Biochemistry Environmental Chemistry Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry Electrochemistry |
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