NDLI: Lateral Shear Forces Applied to Cells with Single Elastic Micropillars to Influence Focal Adhesion Dynamics

Please wait, while we are loading the content...

Lateral Shear Forces Applied to Cells with Single Elastic Micropillars to Influence Focal Adhesion Dynamics

Content Provider	PubMed Central
Author	Heil, Patrick Spatz, Joachim P.
Abstract	Focal adhesions (FAs) are important adhesion sites between eukaryotic cells and the extracellular matrix, their size depending on the locally applied force. To quantitatively study the mechanosensitivity of FAs, we induce their growth and disassembly by varying the distribution of intracellular stress. We present a novel method for micromanipulation of living cells to explore the dynamics of focal adhesion (FA) assembly under force. Fibroblasts are sheared laterally to their adhesion surface with single PDMS micropillars in order to apply laterally stretch or compression to focal adhesions. This allows for measuring the shear force exerted by the micropillar and correlates it with FA length and growth velocity. Furthermore, we analyze the resulting dynamics of FA molecules (paxillin) and compare intensity profiles along FAs before and after the application of external force. The responses of stretched and relaxed FAs differ fundamentally: Relaxed and compressed FAs disassemble isotropically and show no length variation while stretched FAs grow unisotropically in the direction of the applied force and show protein influx only at their front.
Related Links	http://dx.doi.org/10.1088/0953-8984/22/19/194108
File Format	PDF
ISSN	09538984
e-ISSN	1361648X
Journal	Journal of physics. Condensed matter : an Institute of Physics journal
Issue Number	19
Volume Number	22
Language	English
Publisher Date	2010-05-19
Access Restriction	Open
Subject Keyword	Materials Science(all) Condensed Matter Physics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Condensed Matter Physics Materials Science

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in