NDLI: Genomic Survey of Pathogenicity Determinants and VNTR Markers in the Cassava Bacterial Pathogen Xanthomonas axonopodis pv. Manihotis Strain CIO151

Content Provider	PubMed Central
Author	Arrieta-ortiz, Mario L. Rodríguez-r, Luis M. Pérez-quintero, Álvaro L. Poulin, Lucie Díaz, Ana C. Rojas, Nathalia Arias Trujillo, Cesar Benavides, Mariana Restrepo Bart, Rebecca Boch, Jens Tristan, Boureau Darrasse, Armelle David, Perrine Bernonville, Thomas Dugé De Fontanilla, Paula Gagnevin, Lionel Guérin, Fabien Jacques, Marie-agnès Lauber, Emmanuelle Lefeuvre, Pierre Medina, Cesar Medina, Edgar Montenegro, Nathaly Bodnar, Alejandra Muñoz Noël, Laurent D. Quiñones, Juan F. Ortiz Osorio, Daniela Pardo, Carolina Patil, Prabhu B. Stéphane, Poussier Pruvost, Olivier Isabelle, Robène-soustrade Ryan, Robert P. Javier, Tabima Morales, Oscar G. Urrego Christian, Vernière Carrere, Sébastien Verdier, Valérie Boris, Szurek Restrepo, Silvia López, Camilo Ralf, Koebnik Bernal, Adriana
Editor	Arnold, Dawn
Copyright Year	2013
Abstract	Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of bacterial blight of cassava, which is among the main components of human diet in Africa and South America. Current information about the molecular pathogenicity factors involved in the infection process of this organism is limited. Previous studies in other bacteria in this genus suggest that advanced draft genome sequences are valuable resources for molecular studies on their interaction with plants and could provide valuable tools for diagnostics and detection. Here we have generated the first manually annotated high-quality draft genome sequence of Xam strain CIO151. Its genomic structure is similar to that of other xanthomonads, especially Xanthomonas euvesicatoria and Xanthomonas citri pv. citri species. Several putative pathogenicity factors were identified, including type III effectors, cell wall-degrading enzymes and clusters encoding protein secretion systems. Specific characteristics in this genome include changes in the xanthomonadin cluster that could explain the lack of typical yellow color in all strains of this pathovar and the presence of 50 regions in the genome with atypical nucleotide composition. The genome sequence was used to predict and evaluate 22 variable number of tandem repeat (VNTR) loci that were subsequently demonstrated as polymorphic in representative Xam strains. Our results demonstrate that Xanthomonas axonopodis pv. manihotis strain CIO151 possesses ten clusters of pathogenicity factors conserved within the genus Xanthomonas. We report 126 genes that are potentially unique to Xam, as well as potential horizontal transfer events in the history of the genome. The relation of these regions with virulence and pathogenicity could explain several aspects of the biology of this pathogen, including its ability to colonize both vascular and non-vascular tissues of cassava plants. A set of 16 robust, polymorphic VNTR loci will be useful to develop a multi-locus VNTR analysis scheme for epidemiological surveillance of this disease.
Related Links	http://dx.doi.org/10.1371/journal.pone.0079704
Starting Page	79704
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	11
Volume Number	8
Language	English
Publisher	Public Library of Science
Publisher Date	2013-11-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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