NDLI: Molecular Identification and Expressive Characterization of an Olfactory Co-Receptor Gene in the Asian Honeybee, Apis cerana cerana

Content Provider	PubMed Central
Author	Zhao, Huiting Gao, Pengfei Zhang, Chunxiang Ma, Weihua Jiang, Yusuo
Copyright Year	2013
Abstract	Olfaction recognition process is extraordinarily complex in insects, and the olfactory receptors play an important function in the process. In this paper, a highly conserved olfactory co-receptor gene, AcerOr2 (ortholog to the Drosophila melanogaster Or83b), cloned from the antennae of the Asian honeybee, Apis cerana cerana Fabricius (Hymenoptera: Apidae), using reverse transcriptase PCR and rapid amplification of cDNA ends. The full-length sequence of the gene was 1763 bp long, and the cDNA open reading frame encoded 478 amino acid residues, including 7 putative transmembrane domains. Alignment analysis revealed that AcerOr2 shares high homology (> 74%) with similar olfactory receptors found in other Hymenoptera species. The amino acid identity with the closely related species Apis mellifera reached 99.8%. The developmental expression analysis using quantitative real-time reverse transcriptase PCR suggested that the AcerOr2 transcript was expressed at a relatively low level in the larval stage, whereas it was expressed broadly in the pupal and adult stages, with a significantly high level on the days just before and after eclosion. In situ hybridization showed that AcerOr2 mRNA was expressed in sensilla placodea and on the basal region of the worker antennal cuticle, in accordance with the previous conclusions that the conserved genes are expressed in most olfactory receptor neurons.
Related Links	http://dx.doi.org/10.1673/031.013.8001
Starting Page	80
File Format	PDF
ISSN	15362442
e-ISSN	15362442
Journal	Journal of Insect Science
Volume Number	13
Language	English
Publisher	University of Wisconsin Library
Publisher Date	2013-08-01
Access Restriction	Open
Rights Holder	University of Wisconsin Library
Subject Keyword	Insect Science Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Insect Science

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