NDLI: Later Passages of Neural Progenitor Cells from Neonatal Brain Are More Permissive for Human Cytomegalovirus Infection

Content Provider	PubMed Central
Author	Pan, Xing Li, Xiao-jun Liu, Xi-juan Yuan, Hui Li, Jia-fu Duan, Ying-liang Ye, Han-qing Fu, Ya-ru Qiao, Guan-hua Wu, Cong-cong Yang, Bo Tian, Xiao-hui Hu, Kang-hong Miao, Ling-feng Chen, Xiao-ling Zheng, Jun Rayner, Simon Schwartz, Philip H. Britt, William J. Xu, Jiang Luo, Min-hua
Copyright Year	2013
Abstract	Congenital human cytomegalovirus (HCMV) infection is the most frequent infectious cause of birth defects, primarily neurological disorders. Neural progenitor/stem cells (NPCs) are the major cell type in the subventricular zone and are susceptible to HCMV infection. In culture, the differentiation status of NPCs may change with passage, which in turn may alter susceptibility to virus infection. Previously, only early-passage (i.e., prior to passage 9) NPCs were studied and shown to be permissive to HCMV infection. In this study, NPC cultures derived at different gestational ages were evaluated after short (passages 3 to 6) and extended (passages 11 to 20) in vitro passages for biological and virological parameters (i.e., cell morphology, expression of NPC markers and HCMV receptors, viral entry efficiency, viral gene expression, virus-induced cytopathic effect, and release of infectious progeny). These parameters were not significantly influenced by the gestational age of the source tissues. However, extended-passage cultures showed evidence of initiation of differentiation, increased viral entry, and more efficient production of infectious progeny. These results confirm that NPCs are fully permissive for HCMV infection and that extended-passage NPCs initiate differentiation and are more permissive for HCMV infection. Later-passage NPCs being differentiated and more permissive for HCMV infection suggest that HCMV infection in fetal brain may cause more neural cell loss and give rise to severe neurological disabilities with advancing brain development.
Related Links	http://dx.doi.org/10.1128/jvi.01120-13
Ending Page	10979
Page Count	12
Starting Page	10968
File Format	PDF
ISSN	0022538X
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	20
Volume Number	87
Language	English
Publisher	American Society for Microbiology
Publisher Date	2013-10-15
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Immunology Virology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

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