NDLI: Induction and Analysis of the Alkaloid Mitragynine Content of a Mitragyna speciosa Suspension Culture System upon Elicitation and Precursor Feeding

Content Provider	PubMed Central
Author	Mohamad, Zuldin Nor Nahazima Said, Ikram Md. Mdnoor, Normah Zainal, Zamri Jin, Kiat Chew Ismail, Ismanizan
Copyright Year	2013
Abstract	This study aimed to determine the effects of different concentrations and combinations of the phytohormones 2,4-dichlorophenoxy acetic acid (2,4-D), kinetin, 6-benzylaminopurine(BAP), and 1-naphthaleneacetic acid (NAA) on callus induction and to demonstrate the role of elicitors and exogenous precursors on the production of mitragynine in a Mitragyna speciosa suspension culture.The best callus induction was achieved from petiole explants cultured on WPM that was supplemented with 4 mg L−1 2, 4-D (70.83%). Calli were transferred to liquid media and agitated on rotary shakers to establishMitragyna speciosa cell suspension cultures. The optimum settled cell volume was achieved in the presence of WPM that contained 3 mg L−1 2,4-Dand 3% sucrose (9.47 ± 0.4667 mL). The treatment of cultures withdifferent concentrations of yeast extract and salicylic acid for different inoculation periods revealed that the highest mitragynine content as determined by HPLC was achieved from the culture treated with 250 mg L−1 yeast extract (9.275 ± 0.082 mg L−1) that was harvested on day 6 of culturing; salicylic acid showed low mitragynine content in all concentrations used. Tryptophan and loganin were used as exogenous precursors;the highest level of mitragynine production was achieved in cultures treated with 3 μ M tryptophan and harvested at 6 days (13.226 ± 1.98 mg L−1).
Related Links	http://dx.doi.org/10.1155/2013/209434
Starting Page	209434
File Format	PDF
ISSN	1537744X
e-ISSN	1537744X
Journal	The Scientific World Journal
Volume Number	2013
Language	English
Publisher	Hindawi Publishing Corporation
Publisher Date	2013-08-26
Access Restriction	Open
Rights Holder	Hindawi Publishing Corporation
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry, Genetics and Molecular Biology Environmental Science

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