NDLI: Synapto-Protective Drugs Evaluation in Reconstructed Neuronal Network

Content Provider	PubMed Central
Author	Deleglise, Bérangère Lassus, Benjamin Soubeyre, Vaneyssa Alleaume-butaux, Aurélie Hjorth, Johannes J. Vignes, Maéva Schneider, Benoit Bernard, Brugg Viovy, Jean-louis Peyrin, Jean-michel
Editor	Guillemin, Gilles J.
Copyright Year	2013
Abstract	Chronic neurodegenerative syndromes such as Alzheimer’s and Parkinson’s diseases, or acute syndromes such as ischemic stroke or traumatic brain injuries are characterized by early synaptic collapse which precedes axonal and neuronal cell body degeneration and promotes early cognitive impairment in patients. Until now, neuroprotective strategies have failed to impede the progression of neurodegenerative syndromes. Drugs preventing the loss of cell body do not prevent the cognitive decline, probably because they lack synapto-protective effects. The absence of physiologically realistic neuronal network models which can be easily handled has hindered the development of synapto-protective drugs suitable for therapies. Here we describe a new microfluidic platform which makes it possible to study the consequences of axonal trauma of reconstructed oriented mouse neuronal networks. Each neuronal population and sub-compartment can be chemically addressed individually. The somatic, mid axon, presynaptic and postsynaptic effects of local pathological stresses or putative protective molecules can thus be evaluated with the help of this versatile “brain on chip” platform. We show that presynaptic loss is the earliest event observed following axotomy of cortical fibers, before any sign of axonal fragmentation or post-synaptic spine alteration. This platform can be used to screen and evaluate the synapto-protective potential of several drugs. For instance, NAD+ and the Rho-kinase inhibitor Y27632 can efficiently prevent synaptic disconnection, whereas the broad-spectrum caspase inhibitor zVAD-fmk and the stilbenoid resveratrol do not prevent presynaptic degeneration. Hence, this platform is a promising tool for fundamental research in the field of developmental and neurodegenerative neurosciences, and also offers the opportunity to set up pharmacological screening of axon-protective and synapto-protective drugs.
Related Links	http://dx.doi.org/10.1371/journal.pone.0071103
Starting Page	71103
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	8
Volume Number	8
Language	English
Publisher	Public Library of Science
Publisher Date	2013-08-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Synapto-protective drugs evaluation in reconstructed neuronal network

Control of Neuronal Network in Caenorhabditis elegans

NT2 Derived Neuronal and Astrocytic Network Signalling

Bifurcations of Emergent Bursting in a Neuronal Network

Computational approaches to neuronal network analysis

Automated Detection of Soma Location and Morphology in Neuronal Network Cultures

Complexities and uncertainties of neuronal network function

Neuronal Correlates of a Virtual-Reality-Based Passive Sensory P300 Network

Characterizing the Network of Drugs and Their Affected Metabolic Subpathways

Synapto-Protective Drugs Evaluation in Reconstructed Neuronal Network

Similar Documents

Synapto-protective drugs evaluation in reconstructed neuronal network

Control of Neuronal Network in Caenorhabditis elegans

NT2 Derived Neuronal and Astrocytic Network Signalling

Bifurcations of Emergent Bursting in a Neuronal Network

Computational approaches to neuronal network analysis

Automated Detection of Soma Location and Morphology in Neuronal Network Cultures

Complexities and uncertainties of neuronal network function

Neuronal Correlates of a Virtual-Reality-Based Passive Sensory P300 Network

Characterizing the Network of Drugs and Their Affected Metabolic Subpathways

Synapto-Protective Drugs Evaluation in Reconstructed Neuronal Network