NDLI: The quantal nature of Ca2+ sparks and in situ operation of the ryanodine receptor array in cardiac cells

Content Provider	PubMed Central
Author	Wang, Shi Qiang Stern, Michael D. Ríos, Eduardo Cheng, Heping
Copyright Year	2004
Abstract	Intracellular Ca2+ release in many types of cells is mediated by ryanodine receptor Ca2+ release channels (RyRCs) that are assembled into two-dimensional paracrystalline arrays in the endoplasmic/sarcoplasmic reticulum. However, the in situ operating mechanism of the RyRC array is unknown. Here, we found that the elementary Ca2+ release events, Ca2+ sparks from individual RyRC arrays in rat ventricular myocytes, exhibit quantized Ca2+ release flux. Analysis of the quantal property of Ca2+ sparks provided a view of unitary Ca2+ current and gating kinetics of the RyRC in intact cells and revealed that spark activation involves dynamic recruitment of small, variable cohorts of RyRCs. Intriguingly, interplay of RyRCs in multichannel sparks renders an unusual, thermodynamically irreversible mode of channel gating that is unshared by an RyRC acting solo, nor by RyRCs in vitro. Furthermore, an array-based inhibitory feedback, overriding the regenerative Ca2+-induced Ca2+ release of RyRCs, provides a supramolecular mechanism for the microscopic stability of intracellular Ca2+ signaling.
Related Links	http://dx.doi.org/10.1073/pnas.0306157101
Ending Page	3984
Page Count	6
Starting Page	3979
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	11
Volume Number	101
Language	English
Publisher	National Academy of Sciences
Publisher Date	2004-03-16
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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The quantal nature of $Ca^{2+}$ sparks and in situ operation of the ryanodine receptor array in cardiac cells

Ca2+ sparks operated by membrane depolarization require isoform 3 ryanodine receptor channels in skeletal muscle

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Ryanodine Receptor Current Amplitude Controls Ca2+ Sparks in Cardiac Muscle

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Data from: Effects of rogue ryanodine receptors on Ca2+ sparks in cardiac myocytes (Dataset)

The quantal nature of Ca2+ sparks and in situ operation of the ryanodine receptor array in cardiac cells

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The quantal nature of $Ca^{2+}$ sparks and in situ operation of the ryanodine receptor array in cardiac cells

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Ryanodine sensitizes the cardiac Ca2+ release channel (ryanodine receptor isoform 2) to Ca2+ activation and dissociates as the channel is closed by Ca2+ depletion

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