NDLI: Formation of cyclobutane pyrimidine dimers at dipyrimidines containing 5-hydroxymethylcytosine

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Formation of cyclobutane pyrimidine dimers at dipyrimidines containing 5-hydroxymethylcytosine

Content Provider	PubMed Central
Author	Kim, Sang-in Jin, Seung-gi Pfeifer, Gerd P.
Abstract	Much of the cancer-causing effects of ultraviolet radiation from the sun have been linked to the formation of dimerized DNA bases. These dimeric DNA photoproducts include the cyclobutane pyrimidine dimers (CPDs) and the pyrimidine(6–4)pyrimidone photoproducts [(6–4)PPs). CPDs are highly mutagenic and are produced in substantial quantities by UVB radiation. These dimers can form between any two adjacent pyrimidines and can involve thymine, cytosine, or 5-methylcytosine. Very recently, a sixth DNA base, 5-hydroxymethylcytosine (5hmC) has been identified and characterized as a normal component of mammalian DNA. Here, we investigated the formation of CPDs at different DNA sequences containing 5hmC following irradiation with UVA, UVB, or UVC light sources. We show that the formation of CPDs at dipyrimidines containing 5hmC occurs at different DNA sequences but is not enhanced relative to cytosine or 5-methylcytosines at the same sequence positions. In fact, in some sequence contexts, CPDs containing 5hmC are formed at very low levels. Nonetheless, CPD formation at 5hmC pyrimidines is expected to be biologically relevant since three types of human skin-derived cells, fibroblasts, keratinocytes and melanocytes, all contain detectable levels of this modified base.
Related Links	http://dx.doi.org/10.1039/c3pp50037c
Ending Page	1415
Page Count	7
Starting Page	1409
File Format	PDF
ISSN	1474905X
e-ISSN	14749092
Journal	Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
Issue Number	8
Volume Number	12
Language	English
Publisher Date	2013-01-01
Access Restriction	Open
Subject Keyword	Physical and Theoretical Chemistry Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry

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UVA-induced cyclobutane pyrimidine dimers form predominantly at thymine–thymine dipyrimidines and correlate with the mutation spectrum in rodent cells

UVA‐induced cyclobutane pyrimidine dimers form predominantly at thymine–thymine dipyrimidines and correlate with the mutation spectrum in rodent cells

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Generation of oligonucleotides containing site-specific cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidinone photoproducts by immunoprecipitation.

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Theoretical Insight into the Intrinsic Ultrafast Formation of Cyclobutane Pyrimidine Dimers in UV-Irradiated DNA: Thymine versus Cytosine

Formation of cyclobutane pyrimidine dimers at dipyrimidines containing 5-hydroxymethylcytosine

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