NDLI: The mechanism of splenic iNKT cell activation dictates localization in vivo

Content Provider	PubMed Central
Author	King, Irah L. Amiel, Eyal Tighe, Mike Mohrs, Katja Veerapen, Natacha Besra, Gurdyal Markus, Mohrs Leadbetter, Elizabeth A.
Abstract	Invariant Natural Killer T (iNKT) cells are glycolipid-specific innate lymphocytes emerging as critical players in the immune response to diverse infections and disease. iNKT cells are activated either through cognate interactions with lipid-loaded antigen-presenting cells, by antigen-independent cytokine-mediated signaling pathways, or a combination of both. While each of these modes of iNKT cell activation play important roles in directing the humoral and cell-mediated immune response, the spatio-temporal nature of these interactions and the cellular requirements for activation are largely undefined. Combining novel in situ confocal imaging of αGalactosylceramide-loaded CD1d tetramer labeling to localize the endogenous iNKT cell population with cytokine reporter mice, we reveal the choreography of early murine splenic iNKT cell activation across diverse settings of glycolipid immunization and systemic infection with Streptococcus pneumoniae. We find that iNKT cells consolidate in the marginal zone and require dendritic cells lining the splenic marginal zone for activation following administration of cognate glycolipids and during systemic infection but not following exogenous cytokine administration. While further establishing the importance of cognate iNKT cell interactions with antigen-presenting cells, we also show that non-cognate iNKT-dependent mechanisms are sufficient to mediate effector outcomes such as STAT signaling and DC licensing throughout the entire splenic parenchyma. Collectively, these data provide new insight into how iNKT cells may serve as a natural adjuvant in facilitating adaptive immune responses irrespective of their tissue localization.
Related Links	http://dx.doi.org/10.4049/jimmunol.1300299
Ending Page	582
Page Count	11
Starting Page	572
File Format	PDF
ISSN	15506606
e-ISSN	15506606
Journal	Journal of immunology (Baltimore, Md. : 1950)
Issue Number	2
Volume Number	191
Language	English
Publisher Date	2013-07-15
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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