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| Content Provider | PubMed Central |
|---|---|
| Author | Contreras-naranjo, Jose C. Ugaz, Victor M. |
| Copyright Year | 2013 |
| Abstract | Interferometric techniques have proven useful to infer proximity and local surface profiles of microscopic objects near surfaces. But a critical trade-off emerges between accuracy and mathematical complexity when these methods are applied outside the vicinity of closest approach. Here we introduce a significant advancement that enables reflection interference contrast microscopy to provide nearly instantaneous reconstruction of an arbitrary convex object’s contour next to a bounding surface with nanometre resolution, making it possible to interrogate microparticle/surface interaction phenomena at radii of curvature 1,000 times smaller than those accessible by the conventional surface force apparatus. The unique view-from-below perspective of reflection interference contrast microscopy also reveals previously unseen deformations and allows the first direct observation of femtolitre-scale capillary condensation dynamics underneath micron-sized particles. Our implementation of reflection interference contrast microscopy provides a generally applicable nanometre-scale resolution tool that can be potentially exploited to dynamically probe ensembles of objects near surfaces so that statistical/probabilistic behaviour can be realistically captured. |
| Related Links | http://dx.doi.org/10.1038/ncomms2865 |
| Starting Page | 1919 |
| File Format | |
| ISSN | 20411723 |
| e-ISSN | 20411723 |
| Journal | Nature Communications |
| Volume Number | 4 |
| Language | English |
| Publisher | Nature Pub. Group |
| Publisher Date | 2013-05-28 |
| Access Restriction | Open |
| Rights Holder | Nature Pub. Group |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Physics and Astronomy Biochemistry, Genetics and Molecular Biology |
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