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| Content Provider | PubMed Central |
|---|---|
| Author | Frankowski, Marcin Theisen, Janko Andreas, Kummrow Simon, Peter Ragusch, Hülya Bock, Nicole Schmidt, Martin Jörg, Neukammer |
| Copyright Year | 2013 |
| Abstract | This study demonstrates the suitability of microfluidic structures for high throughput blood cell analysis. The microfluidic chips exploit fully integrated hydrodynamic focusing based on two different concepts: Two-stage cascade focusing and spin focusing (vortex) principle. The sample—A suspension of micro particles or blood cells—is injected into a sheath fluid streaming at a substantially higher flow rate, which assures positioning of the particles in the center of the flow channel. Particle velocities of a few m/s are achieved as required for high throughput blood cell analysis. The stability of hydrodynamic particle positioning was evaluated by measuring the pulse heights distributions of fluorescence signals from calibration beads. Quantitative assessment based on coefficient of variation for the fluorescence intensity distributions resulted in a value of about 3% determined for the micro-device exploiting cascade hydrodynamic focusing. For the spin focusing approach similar values were achieved for sample flow rates being 1.5 times lower. Our results indicate that the performances of both variants of hydrodynamic focusing suit for blood cell differentiation and counting. The potential of the micro flow cytometer is demonstrated by detecting immunologically labeled CD3 positive and CD4 positive T-lymphocytes in blood. |
| Related Links | http://dx.doi.org/10.3390/s130404674 |
| Ending Page | 4693 |
| Page Count | 20 |
| Starting Page | 4674 |
| File Format | |
| ISSN | 14248220 |
| e-ISSN | 14248220 |
| Journal | Sensors (Basel, Switzerland) |
| Issue Number | 4 |
| Volume Number | 13 |
| Language | English |
| Publisher | Molecular Diversity Preservation International (MDPI) |
| Publisher Date | 2013-04-01 |
| Access Restriction | Open |
| Rights Holder | Molecular Diversity Preservation International (MDPI) |
| Subject Keyword | Electrical and Electronic Engineering Analytical Chemistry Atomic and Molecular Physics, and Optics Biochemistry Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Atomic and Molecular Physics, and Optics Instrumentation Analytical Chemistry Biochemistry Information Systems Electrical and Electronic Engineering |
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