NDLI: Cell-dependent efficiency of reiterated nuclear signals in a mutant simian virus 40 oncoprotein targeted to the nucleus.

Please wait, while we are loading the content...

Year: 2016, Volume: 36

Year: 2015, Volume: 36

Year: 2015, Volume: 35

Year: 2014, Volume: 35

Year: 2014, Volume: 34

Year: 2013, Volume: 33

Year: 2012, Volume: 32

Year: 2011, Volume: 31

Year: 2010, Volume: 30

Year: 2009, Volume: 29

Year: 2008, Volume: 28

Year: 2007, Volume: 27

Year: 2006, Volume: 26

Year: 2005, Volume: 25

Year: 2004, Volume: 24

Year: 2003, Volume: 23

Year: 2002, Volume: 23

Year: 2002, Volume: 22

Year: 2001, Volume: 21

Year: 2000, Volume: 20

Year: 1999, Volume: 19

Year: 1998, Volume: 18

Year: 1997, Volume: 17

Year: 1996, Volume: 16

Year: 1995, Volume: 15

Year: 1994, Volume: 14

Year: 1993, Volume: 13

Year: 1992, Volume: 12

Year: 1991, Volume: 11

Year: 1990, Volume: 10

Year: 1989, Volume: 9

Year: 1988, Volume: 8

Year: 1988, Volume: 8, Issue: 12

Dynamic changes in the structure and intracellular locale of the mammalian low-molecular-weight heat shock protein.

Fast skeletal muscle-specific expression of a quail troponin I gene in transgenic mice.

P21 v-ras inhibits induction of c-myc and c-fos expression by platelet-derived growth factor.

Connections between transcriptional activators, silencers, and telomeres as revealed by functional analysis of a yeast DNA-binding protein.

Isolation and characterization of the human chorionic gonadotropin beta subunit (CG beta) gene cluster: regulation of transcriptionally active CG beta gene by cyclic AMP.

The intron-containing gene for yeast profilin (PFY) encodes a vital function.

Expression of human adenosine deaminase in murine hematopoietic cells.

Role of tyrosine kinase and membrane-spanning domains in signal transduction by the platelet-derived growth factor receptor.

SAM2 encodes the second methionine S-adenosyl transferase in Saccharomyces cerevisiae: physiology and regulation of both enzymes.

Isolation of a yeast gene involved in species-specific pre-tRNA processing.

Mitochondrial import and processing of mutant human ornithine transcarbamylase precursors in cultured cells.

Identification of protein-binding sites in the hepatitis B virus enhancer and core promoter domains.

Evidence for cooperation between cells during sporulation of the yeast Saccharomyces cerevisiae.

Interaction of a hepatoma-specific nuclear factor with transcription-regulatory sequences of the human alpha-fetoprotein and albumin genes.

Amplified DNAs in laboratory stocks of Leishmania tarentolae: extrachromosomal circles structurally and functionally similar to the inverted-H-region amplification of methotrexate-resistant Leishmania major.

Differential regulation of the two glyceraldehyde-3-phosphate dehydrogenase genes during Drosophila development.

The brown protein of Drosophila melanogaster is similar to the white protein and to components of active transport complexes.

Novel B-cell precursors blocked at the stage of DJH recombination.

The development expression of the rat alpha-vascular and gamma-enteric smooth muscle isoactins: isolation and characterization of a rat gamma-enteric actin cDNA.

Functional interaction between transcriptional elements in the long terminal repeat of reticuloendotheliosis virus: cooperative DNA binding of promoter- and enhancer-specific factors.

Ty3, a yeast retrotransposon associated with tRNA genes, has homology to animal retroviruses.

Stepwise activation of the mouse acetylcholine receptor delta- and gamma-subunit genes in clonal cell lines.

The dihydrofolate reductase amplicons in different methotrexate-resistant Chinese hamster cell lines share at least a 273-kilobase core sequence, but the amplicons in some cell lines are much larger and are remarkably uniform in structure.

Transcriptional and posttranscriptional mechanisms regulate murine thymidine kinase gene expression in serum-stimulated cells.

Gene conversion adjacent to regions of double-strand break repair.

A Ty1 cell-type-specific regulatory sequence is a recognition element for a constitutive binding factor.

Nuclear proteins that bind the human gamma-globin gene promoter: alterations in binding produced by point mutations associated with hereditary persistence of fetal hemoglobin.

Estrogen and progesterone receptor-binding sites on the chicken vitellogenin II gene: synergism of steroid hormone action.

Differential repair of DNA damage in the human metallothionein gene family.

Regulatory myosin light-chain genes of Caenorhabditis elegans.

Dependence of intrachromosomal recombination in mammalian cells on uninterrupted homology.

Expression of v-rel induces mature B-cell lines that reflect the diversity of avian immunoglobulin heavy- and light-chain rearrangements.

Transcription termination at the chicken beta H-globin gene.

In situ localization of the per clock protein during development of Drosophila melanogaster.

A yeast gene essential for regulation of spindle pole duplication.

Matrix attachment regions are positioned near replication initiation sites, genes, and an interamplicon junction in the amplified dihydrofolate reductase domain of Chinese hamster ovary cells.

Role of SGP2, a suppressor of a gpa1 mutation, in the mating-factor signaling pathway of Saccharomyces cerevisiae.

Cloning of the PYR3 gene of Ustilago maydis and its use in DNA transformation.

Determination of the spectrum of mutations induced by defined-wavelength solar UVB (313-nm) radiation in mammalian cells by use of a shuttle vector.

Prolactin upstream factor I mediates cell-specific transcription.

The first and fourth upstream open reading frames in GCN4 mRNA have similar initiation efficiencies but respond differently in translational control to change in length and sequence.

Inhibition of T-cell antigen receptor-mediated transmembrane signaling by protein kinase C activation.

Expression of T-cell receptor alpha-chain genes in transgenic mice.

Expression and regulation of the pituitary- and placenta-specific human glycoprotein hormone alpha-subunit gene is restricted to the pituitary in transgenic mice.

The epidermal growth factor receptor from prostate cells is dephosphorylated by a prostate-specific phosphotyrosyl phosphatase.

The major inducible heat shock protein hsp68 is not required for acquisition of thermal resistance in mouse plasmacytoma cell lines.

Cell-dependent efficiency of reiterated nuclear signals in a mutant simian virus 40 oncoprotein targeted to the nucleus.

Silk gland-specific tRNA(Ala) genes are tightly clustered in the silkworm genome.

The chromatin structure at the promoter of a glyceraldehyde phosphate dehydrogenase gene from Saccharomyces cerevisiae reflects its functional state.

Activation of the transforming potential of the human fos proto-oncogene requires message stabilization and results in increased amounts of partially modified fos protein.

Genetics and polymorphism of the mouse prion gene complex: control of scrapie incubation time.

Novel tyrosine kinase identified by phosphotyrosine antibody screening of cDNA libraries.

Effect of c-myc gene expression on early inducible reactions required for erythroid differentiation in vitro.

The human transforming growth factor alpha promoter directs transcription initiation from a single site in the absence of a TATA sequence.

Multistress resistance of Saccharomyces cerevisiae is generated by insertion of retrotransposon Ty into the 5' coding region of the adenylate cyclase gene.

Isolation and characterization of a cDNA that encodes mouse fibroblast tropomyosin isoform 2.

Developmental and tissue-specific expression of U4 small nuclear RNA genes.

Different structural alterations upregulate in vitro tyrosine kinase activity and transforming potency of the erbB-2 gene.

U2 small nuclear RNA is remarkably conserved between Schizosaccharomyces pombe and mammals.

T-cell activation signals and human T-cell leukemia virus type I-encoded p40x protein activate the mouse granulocyte-macrophage colony-stimulating factor gene through a common DNA element.

Tumor necrosis factor suppresses transcription of the thrombomodulin gene in endothelial cells.

Murine Hox-1.7 Homeo-Box Gene: Cloning, Chromosomal Location, and Expression

Year: 1988, Volume: 8, Issue: 11

Year: 1988, Volume: 8, Issue: 10

Year: 1988, Volume: 8, Issue: 9

Year: 1988, Volume: 8, Issue: 8

Year: 1988, Volume: 8, Issue: 7

Year: 1988, Volume: 8, Issue: 6

Year: 1988, Volume: 8, Issue: 5

Year: 1988, Volume: 8, Issue: 4

Year: 1988, Volume: 8, Issue: 3

Year: 1988, Volume: 8, Issue: 2

Year: 1988, Volume: 8, Issue: 1

Year: 1987, Volume: 7

Year: 1986, Volume: 6

Year: 1985, Volume: 5

Year: 1984, Volume: 4

Year: 1983, Volume: 3

Year: 1982, Volume: 2

Year: 1981, Volume: 1

Cell-dependent efficiency of reiterated nuclear signals in a mutant simian virus 40 oncoprotein targeted to the nucleus.

Content Provider	PubMed Central
Author	Fischer-fantuzzi, L. Vesco, C.
Abstract	We investigated the requisites for, and functional consequences of, the relocation to the nucleus of a transforming nonkaryophilic mutant of the simian virus 40 large T antigen (a natural deletion mutant lacking an internal large-T-antigen domain that includes the signal for nuclear transport). Synthetic oligonucleotides were used to obtain gene variants with one or more copies of the signal-specifying sequence inserted near the gene 3' end, in a region dispensable for the main large-T-antigen functions. The analysis of stable transfectant populations showed that mouse NIH 3T3 cells, rat embryo fibroblasts, and simian CS cells (a subclone of CV1 cells) differed considerably in their ability to localize some variant molecules into the nucleus. CS cells were always the most efficient, and NIH 3T3 cells were the least efficient. The nuclear localization improved either with reiteration of the signal or with a left-flank modification of the signal amino acid context. Three signals appeared to be necessary and sufficient, even in NIH 3T3 cells, to obtain a nuclear accumulation comparable to that of wild-type simian virus 40 large T antigen; other signal-cell combinations caused a large variability in subcellular localization among cells of the same population, as if the nuclear uptake of some molecules depended on individual cell states. The effect of the modified location on the competence of the protein to alter cell growth was examined by comparing the activity of variants containing either the normal signal or a signal with a mutation (corresponding to large-T-antigen amino acid 128) that prevented nuclear transport. It was found that the nuclear variant was slightly more active than the cytoplasmic variants in rat embryo fibroblasts and NIH 3T3 cells and was notably less active in CS cells.
Starting Page	5495
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	12
Volume Number	8
Language	English
Publisher Date	1988-12-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in